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EMAGE:16169

Mir320 microRNA 320 ( MGI:3619332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169
"Pseudo-wholemount" of euxassay_019132. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019132_01 euxassay_019132_02 euxassay_019132_03 euxassay_019132_04
EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169
euxassay_019132_05 euxassay_019132_06 euxassay_019132_07 euxassay_019132_08 euxassay_019132_09
EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169
euxassay_019132_10 euxassay_019132_11 euxassay_019132_12 euxassay_019132_13 euxassay_019132_14
EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169 EMAGE:16169
euxassay_019132_15 euxassay_019132_16 euxassay_019132_17 euxassay_019132_18 euxassay_019132_19
EMAGE:16169
euxassay_019132_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16169Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16169_wholemount_strong.wlz
16169_wholemount_moderate.wlz
16169_wholemount_weak.wlz
16169_wholemount_possible.wlz
16169_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16169_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
humerus
weak weak
regionalweak expression: see section 01 16 17 18 19 20
fibula
weak weak
regionalweak expression: see section 20
tibia
weak weak
regionalweak expression: see section 20
femur
weak weak
regionalweak expression: see section 02 03 04 05 16 17 18
meckel's cartilage
weak weak
regionalweak expression: see section 02 03 04 05 06 07 11 12 13 14 15 16 17
liver lobe
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
scapula
weak weak
regionalweak expression: see section 16 17
pelvic girdle skeleton
weak weak
regionalweak expression: see section 06 13 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70396
Entity Detected:Mir320, microRNA 320 ( MGI:3619332)
Sequence:sense strand is shown

>T70396
AAAAGCTGGGTTGAGAGGGCGA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-320 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16168 same embryo
 EMAGE:16170 same embryo
 EurExpress:euxassay_019132 same experiment
 MGI:4826268 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS