EMAGE mouse gene expression spatial database

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EMAGE:16216

Tmem115 transmembrane protein 115 ( MGI:1930765)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017072. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017072_01	euxassay_017072_02	euxassay_017072_03	euxassay_017072_04

euxassay_017072_05	euxassay_017072_06	euxassay_017072_07	euxassay_017072_08	euxassay_017072_09

euxassay_017072_10	euxassay_017072_11	euxassay_017072_12	euxassay_017072_13	euxassay_017072_14

euxassay_017072_15	euxassay_017072_16	euxassay_017072_17	euxassay_017072_18	euxassay_017072_19

euxassay_017072_20	euxassay_017072_21	euxassay_017072_22	euxassay_017072_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16216_wholemount_strong.wlz

16216_wholemount_moderate.wlz

16216_wholemount_weak.wlz

16216_wholemount_possible.wlz

16216_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16216_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7232
Entity Detected:	Tmem115, transmembrane protein 115 ( MGI:1930765)
Sequence:	sense strand is shown >T7232 CGTCAAGCCAAAACCCTAGCCCAGCCGGAGCTAAACGGGCTTGACTTGGGCCGGAACGAGGCACCAGTTC CCCGCAGATCGCAGAGTCTCAGAGTGGATGGAGGAAGCCTAGCCTTGAGATTAACGCTAGCCTGGCCGCT GGGCCGACGGAACCCGCAGGCAGGCGAGCCCAAGCTACCCAGGGCCTAACGACAGGTCCCCGGCAAGAAG ACTTTCTCCTCGCTTTGGAACTACAACCGGATCAAACCGGAACCAGAGCCTTCCCACGGAACAGAAGCCA GTGAAGTAGCCGGGCCCGGGCCTCCCGTCGGGGCCGAACTGGGACGAGGCCCCGGGGAGGCCCCTAGGCC ACCTTCCACACATTCCCTTAAGCCAACGTCCGCCAGGAAGATGCAACGCGCCCTACCTGGTGCCCGCCAA CATCTGGGGGCCATCCTGGCCAGCGCCAGCGTGGTGGTGAAGGCACTGTGCGCCGTGGTACTGTTTCTCT ACCTGCTTTCCTTCGCTGTGGACACGGGCTGCCTGGCCGTCACCCCAGGCTACCTTTTCCCACCCAACTT CTGGATCTGGACCCTGGCCACCCACGGGCTCATGGAACAGCACGTGTGGGACGTGGCCATTAGCCTGGCC ACAGTGGTTGTGGCCGGGCGATTACTGGAGCCCCTCTGGGGAGCCTTGGAGCTGCTCATCTTCTTCTCGG TGGTGAATGTGTCAGTGGGGCTTCTGGGGGCCCTCGCCTACCTCCTCACCTACATGGCTTCCTTCAACTT GGTTTACCTGTTCACTATTCGTATCCACGGCGCCCTGGGTTTCCTAGGTGGTGTTCTGGTAGCCCTCAAG CAAACTATGGGAGACTGTGTGGTTCTGCGAGTGCCCCAGGTCCGCGTCAGCGTCGTTCCCATGCTGTTGC TGGCGTTGCTGCTACTCCTCCGGTTGGCCACGCTGCTCCAGAGCCCAGCCCTGGCTTCCTACGGCTTTGG GCTGCTATCGAGTTGGGTATATCTTCGCTTCTATCAGCGCCATAGCCGGGGCCGAGGGGACATGGCTGAC CATTTTGCTTTTGCCACCTTCTTCCCGGAGATCCTGCAACCGGTGGTGGGGCTGCTAGCGAACTTGGTGC ACGGCCTCCTGGTGAAAGTAAAGATATGCCAGAAGACAGTGAAGCGCTACGATGTGGGAGCGCCGTCGTC CATCACTATCAGCCTCCCAGGCACAGACCCTCAAGATGCGGAGCGCAGAAGGCAACTAGCCCTAAAGGCT CTCAATGAGCGACTGAAGAGAGTGGAGGATCAGTCAGCCTGGCCCAGCATGGATGATGACGAAGAGGAAG CTGGGGCAAAAACAGACAGTCCTCTGCCCTTAGAAGAAGCTTCCACGCCCCCAGGGAAGGTGACCGTCCC TGAATCCAGTCTCATCACCTTGGAGACAGCTCCCCTACTCTAGACCACTTTGAGTGCAGTTCTTCTACTC CCATGCCTTCCCGATCCGTCTCGGCTACTGCAGAGCACTGACTGCTCTGAGGAGAGGGAAGAGGGTCTGC TGGGGGTTCCTGTGGCCTTCCGCTGTGTCTGGACAACACTAACACAGGACCCTTGTTGCTACCTGGTTCT GACTCCAGACAACCACAATGCCAGGTACGGGGTCTCTGAGCAGCAGCCAGTGCAGATCCCCATTTGCAGT AAGATTGTACCTCAGTGGTACAATTCCTCACCAAGGAAGTGGCTGCAGGGACACTGGTGCCACGGCTCCT GGGCCAGCCCTTATGTCTGAAACTGGTTGCCAACAGCCCCGAGCCAAGGCAAGGATTTGTGTTTGCCAAA AATGTTCTGGGGACCCAGCCAGTGTGGGTCTGAACATCCCCGCCCACCCCCAGACTGCATTCTCATCAGG GTTTCTTGTGCCCTTCTGCTGTGGCAGTGACAACTGTGCCTAGCCGGGGCTGCCACAGCTCCCAGGTATT TTCTACAGGACCATCTGAGCGGGCAGCCAAACCTGCTTCACAGTATCAATAAAGCGGTTCTTTGAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4161444 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4161444 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16219 same embryo
	EMAGE:16217 same embryo
	EMAGE:16220 same embryo
	EMAGE:16218 same embryo
	EMAGE:16215 same embryo
	EurExpress:euxassay_017072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source