EMAGE mouse gene expression spatial database

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EMAGE:16220

Cpt1b carnitine palmitoyltransferase 1b, muscle ( MGI:1098297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017073. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017073_01	euxassay_017073_02	euxassay_017073_03	euxassay_017073_04

euxassay_017073_05	euxassay_017073_06	euxassay_017073_07	euxassay_017073_08	euxassay_017073_09

euxassay_017073_10	euxassay_017073_11	euxassay_017073_12	euxassay_017073_13	euxassay_017073_14

euxassay_017073_15	euxassay_017073_16	euxassay_017073_17	euxassay_017073_18	euxassay_017073_19

euxassay_017073_20	euxassay_017073_21	euxassay_017073_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16220_wholemount_strong.wlz

16220_wholemount_moderate.wlz

16220_wholemount_weak.wlz

16220_wholemount_possible.wlz

16220_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16220_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4401
Entity Detected:	Cpt1b, carnitine palmitoyltransferase 1b, muscle ( MGI:1098297)
Sequence:	sense strand is shown >T4401 GACCCATACACCGACAGAAGCAAACCTGAGCTGTGCTGAATAAACCCCAGGATGGCGGAAGCACACCAGG CAGTAGCTTTCCAGTTCACTGTGACCCCAGACGGGGTCGACTTCCGGCTTAGTCGGGAGGCTCTGAGACA CATCTACCTGTCTGGAATCAACTCCTGGAAGAAACGCCTTATTCGAATCAAGAATGGCATCCTTAGGGGT GTGTACCCTGGCAGCCCCACCAGCTGGCTGGTTGTTGTCATGGCAACAGTTGGTTCCAACTACTGCAAGG TGGACATCTCCATGGGACTGGTCGATTGCATCCAGAGATGCCTCCCGGAAAGGTATGGCCACTTTGGGAC CCCACAGACAGAGGCACTTCTCAGCATGGTCATCTTCTCCACCGGAGTCTGGGCGACAGGCATTTTCTTC TTCCGACAAACCTTGAAGCTGCTGCTCTCCTACCACGGGTGGATGTTCGAGATGCACAGCAAGACCAGCC ATGCCACCAAGATCTGGGCTATCTGTGTCCGTCTCCTGTCCAGCCGGCGGCCCATGCTCTACAGCTTCCA AACGTCACTGCCTAAGCTTCCTGTCCCCAGCGTGCCAGCCACAATTCACCGGTACTTGGATTCTGTGCGG CCCTTATTGGATGATGAAGCATATTACCGCATGGAGACATTGGCCAAGGAATTCCAGGACAAGACTGCCC CCAGGCTGCAGAAATACCTGGTGCTCAAGTCATGGTGGGCAACTAACTATGTGAGTGACTGGTGGGAAGA ATATGTCTACCTCCGAAGCAGGAGCCCCCTCATGGTGAACAGCAACTATTATGCCATGGATTTTGTGCTT ATTAAGAACACAAATGTGCAAGCAGCCCGTCTAGGCAATGCCGTTCACGCCATGATCATGTATCGCCGCA AACTGGACCGTGAAGAGATCAAGCCGGTCATGGCACTGGGTATGGTGCCCATGTGCTCCTACCAGATGGA GAGGATGTTCAACACTACACGCATCCCAGGCAAAGAGACAGACTTGCTACAGCACCTCTCAGAGAGCAGG CACGTGGCTGTCTACCACAAAGGTCGCTTCTTCAAGGTCTGGCTCTATGAGGGCTCGCGCCTGCTCAAGC CCCGAGACCTGGAGATGCAGTTCCAGAGAATCCTCGACGACCCTTCCCCACCTCAGCCTGGGGAGGAGAA GCTGGCAGCCCTCACCGCAGGAGGAAGGGTAGAGTGGGCAGAGGCACGTCAGACCTTCTTCAGCTCTGGC AAGAACAAGATGTCTCTGGACGCCATCGAACGTGCTGCTTTCTTTGTGACCCTGGATGAAGATTCTCATT GCTACAACCCTGACGATGAGACCAGTCTTAGCCTCTACGGCAAAGCCTTGCTCCATGGCAACTGCTATAA CAGGTGGTTTGACAAATCTTTCACCCTTATCTCCTGCAAGAATGGCCTGTTAGGCCTCAACACCGAACAC TCGTGGGCAGACGCGCCCATCATTGGGCACCTCTGGGAGTTTGTCCTGGGCACTGATACCTTTCACCTGG GCTACACGGAGACAGGACACTGTGTGGGTGAGCCAAACACCACGTTGCCACCCCCTCAGCGGCTGCCGTG GGACATTCCCGAGCAGTGCCGGGAAGCCATCGAGAACTCGTACCAAGTAGCCAAGGCACTGGCCGATGAC GTGGAGCTCTACTGCTTCCAGTTCTTACCCTTTGGCAAAGGTCTTATCAAGAAGTGTAGGACCAGCCCCG ACGCTTTTGTGCAGATTGCCCTACAGCTGGCTCATTTCCGGGACAAAGGCAAGTTCTGCCTGACTTACGA GGCCTCGATGACAAGAATGTTCCGAGAGGGGCGGACTGAGACTGTGCGTTCCTGTACCAACGAGTCCGCA GCCTTTGTGCAGGCCATGATGAAGGGGTCCCATAAGAAACAAGACCTCCAAGATCTCTTCCGGAAAGCCT CCGAAAAGCACCAAAACATGTACCGCCTAGCCATGACAGGGGCTGGGATCGACCGGCACCTCTTCTGCCT TTACATCGTCTCCAAGTACTTAGGAGTTAGCTCTCCTTTCCTGGCTGAGGTACTTTCTGAACCCTGGAGC CTCTCCACCAGCCAGATCCCCCAGTTCCAGATCTGCATGTTTGACCCAAAACAGTATCCCAATCATCTGG GTGCTGGAGGTGGCTTTGGTCCCGTGGCGGATGATGGCTACGGGGTCTCTTACATGATCGCAGGAGAAAA CACCATGTTCTTCCACATCTCCAGCAAATACTCAAGTTCAGAGACGAACGCCCAGCGCTTTGGGAACCAC ATCCGCCAAGCACTGTTGGACATCGCCGAACTTTTCAAAATTTCCAAGACAGACAGCTGAGGAAAAGAGA TACCCCAGCTGCCCTCTGGTCCCCACCTGGTGGAAGAAGAGGCCTGTGGCCAGCTCACAGGCATAAGGGG TGGCGTGCACACACGCCCAGTTCGGAGAACAGCTCTTGAGGCAGTGCTCCCCGGAGGAGGCAGTGCTCCC TGGGCAGATACTGCTCCTCTAGGGCCCCCGCTGGAGGTGGGATTGGAGCAGCAGGGGAATTTTGATTTTT TTTTTTTTTTTTTTGGCTTGGTAGATGTTAATAAAAATAAGGCTGTATAATTCTTAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4920625 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4920625 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16219 same embryo
	EMAGE:16217 same embryo
	EMAGE:16216 same embryo
	EMAGE:16218 same embryo
	EMAGE:16215 same embryo
	EurExpress:euxassay_017073 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source