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EMAGE:16270

Slit1 slit homolog 1 (Drosophila) ( MGI:1315203)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270
"Pseudo-wholemount" of euxassay_017127. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017127_01 euxassay_017127_02 euxassay_017127_03 euxassay_017127_04
EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270
euxassay_017127_05 euxassay_017127_06 euxassay_017127_07 euxassay_017127_08 euxassay_017127_09
EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270
euxassay_017127_10 euxassay_017127_11 euxassay_017127_12 euxassay_017127_13 euxassay_017127_14
EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270 EMAGE:16270
euxassay_017127_15 euxassay_017127_16 euxassay_017127_17 euxassay_017127_18 euxassay_017127_19
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euxassay_017127_20 euxassay_017127_21 euxassay_017127_22 euxassay_017127_23 euxassay_017127_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16270Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16270_wholemount_strong.wlz
16270_wholemount_moderate.wlz
16270_wholemount_weak.wlz
16270_wholemount_possible.wlz
16270_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16270_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63326
Entity Detected:Slit1, slit homolog 1 (Drosophila) ( MGI:1315203)
Sequence:sense strand is shown

>T63326
CTCTGTGTTGAGGGCTACAGTGGACAGCTCTGTGAGATCCCACCTGCCCCCAGGAGCTCCTGTGAGGGGA
CGGAGTGCCAGAATGGAGCCAACTGCGTAGACCAGGGAAGCCGGCCCGTATGCCAGTGCCTACCAGGCTT
TGGTGGCCCTGAATGTGAGAAATTGCTCAGTGTCAACTTTGTGGACCGGGACACTTATCTGCAGTTCACT
GACCTGCAGAACTGGCCTCGGGCCAACATCACTCTTCAGGTCTCCACAGCAGAGGACAATGGGATTCTCC
TATATAATGGGGACAATGACCACATTGCAGTTGAGCTGTACCAGGGCCATGTCCGTGTTAGTTATGACCC
AGGCAGCTACCCCAGCTCTGCTATCTACAGCGCTGAGACGATCAACGATGGGCAGTTCCACACCGTTGAG
CTGGTCACCTTCGACCAGATGGTGAACCTCTCCATCGATGGCGGCAGCCCCATGACCATGGACAATTTTG
GCAAGCACTACACCCTCAACAGCGAGGCCCCCCTCTATGTGGGAGGGATGCCTGTGGACGTGAACTCAGC
TGCCTTCCGCCTGTGGCAGATCCTCAATGGTACCAGCTTCCACGGTTGCATCCGGAACCTGTACATCAAC
AATGAACTGCAGGACTTCACCAAGACACAGATGAAGCCAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74777. Forward Primer - name:074777_F_cDNA_Slit1, sequence:CTCTGTGTTGAGGGCTACAGTG; Reverse Primer - name:074777_N_SP6_cDNA_Slit1, sequence:GCCTGGCTTCATCTGTGTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16268 same embryo
 EMAGE:16272 same embryo
 EMAGE:16269 same embryo
 EMAGE:16271 same embryo
 EMAGE:16267 same embryo
 EurExpress:euxassay_017127 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS