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EMAGE:16341

Akap13 A kinase (PRKA) anchor protein 13 ( MGI:2676556)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341
"Pseudo-wholemount" of euxassay_017027. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017027_01 euxassay_017027_02 euxassay_017027_03 euxassay_017027_04
EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341
euxassay_017027_05 euxassay_017027_06 euxassay_017027_07 euxassay_017027_08 euxassay_017027_09
EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341
euxassay_017027_10 euxassay_017027_11 euxassay_017027_12 euxassay_017027_13 euxassay_017027_14
EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341
euxassay_017027_15 euxassay_017027_16 euxassay_017027_17 euxassay_017027_18 euxassay_017027_19
EMAGE:16341 EMAGE:16341 EMAGE:16341 EMAGE:16341
euxassay_017027_20 euxassay_017027_21 euxassay_017027_22 euxassay_017027_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16341Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16341_wholemount_strong.wlz
16341_wholemount_moderate.wlz
16341_wholemount_weak.wlz
16341_wholemount_possible.wlz
16341_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16341_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45620
Entity Detected:Akap13, A kinase (PRKA) anchor protein 13 ( MGI:2676556)
Sequence:sense strand is shown

>T45620
CACAGTGACTTCTAGTGGCAGGTTGGAAGAATGTGGCAGTGGTAAAGCTAGCCTGCCTGAAAGCACAATG
GTGCAGCCAAGCACGCAAGAACTTTGCACCACCCTCTGCCCAGAAGACCCACAAGCTGACACAGTCACTT
CTGACACTGTGAAAAATACCCAGAAATCTGTGGGGGTTTGTCATCTCTGTGTTTCAGATGCAAAGAATCA
AGGAAATGGTTTGAAGCAAGATACACCTTTAACGAACGTGCTTGAGGATGTTCCACGTCTGCCTTCAGTT
GTCTCCCAGACTGAGAAAGAACTAGCGCCAGACCAGGTATCACCTCCAGCCAGCAGTTTCTCTCTGGCAA
GCAGTCCAGAGAGTGAATCAGTAACCAAAGATGACGCACTTTCTCTTGTCCCCTCCCAGAAAGAAAAGGG
AACAGCAACTCCTCAGCTCCATAGAACTACAGCTTGTCGAGATGGCCCAGATGGAAGAGATTTGAGTGAT
ACCGATAAGGTAGGAGACGGGGCCACTGACCCACCTCCATCCTCTGCTGTAGAGCTTCGGACCAGCATGG
GAAATACCAGCCCTGTAGGCATTGGTGGAGAACAGGAGGGTTCCAGCCCAACAGCAACACTTGAAGTGCT
GAGTGACTCTTTACTGCATAACGTGGATAAAGCTGCTTTGGTTTCTGACTTTACTCTGCCTGAAGAAGGT
GTCAGTGTAGTGGTTCCAGAAAGTTCCACAGCTCTGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 202647. Forward Primer - name:202647_F_exon_Akap13, sequence:CACAGTGACTTCTAGTGGCAGG; Reverse Primer - name:202647_N_SP6_exon_Akap13, sequence:CCCAGAGCTGTGGAACTTTCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16340 same embryo
 EMAGE:16343 same embryo
 EMAGE:16342 same embryo
 EMAGE:16338 same embryo
 EMAGE:16339 same embryo
 EurExpress:euxassay_017027 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS