EMAGE mouse gene expression spatial database

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EMAGE:16370

Vsx2 visual system homeobox 2 ( MGI:88401)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017155. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017155_01	euxassay_017155_02	euxassay_017155_03	euxassay_017155_04

euxassay_017155_05	euxassay_017155_06	euxassay_017155_07	euxassay_017155_08	euxassay_017155_09

euxassay_017155_10	euxassay_017155_11	euxassay_017155_12	euxassay_017155_13	euxassay_017155_14

euxassay_017155_15	euxassay_017155_16	euxassay_017155_17	euxassay_017155_18	euxassay_017155_19

euxassay_017155_20	euxassay_017155_21	euxassay_017155_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16370_wholemount_strong.wlz

16370_wholemount_moderate.wlz

16370_wholemount_weak.wlz

16370_wholemount_possible.wlz

16370_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16370_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

single cell

weak expression: see section 09 12

pons ventricular layer

weak

single cell

weak expression: see section 08 09 12 13

ventral grey horn

weak

single cell

weak expression: see section 09 10 11 12

neural retina

moderate

regional

moderate expression: see section 01 weak expression: see section 02 04 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4128
Entity Detected:	Vsx2, visual system homeobox 2 ( MGI:88401)
Sequence:	sense strand is shown >T4128 CCTGGGACCAACTTCACGAAGCGGGGAGCCCGGCGGGGGGGCTTGGGGGAGCAAAAGACCCGCGGCCTCA GCCCCTCCAAAGAACCGGGAGATGACGGGGAAAGCGGGGGAAGCGCTGAGCAAGCCCAAATCCGAGACAG TGGCCAAGAGTACCTCGGGGGGCGCCCCGGCCAGGTGCACAGGGTTCGGCATCCAGGAGATCCTAGGCTT GAACAAGGAGCCTCCCAGCTCCCACCCGCGGGCTGCCCTCGACGGCCTGGCCCCTGGGCATTTGTTAGCT GCGCGCTCCGTTCTCAGCCCGGCAGGAGTGGGCAGCATGGGGCTGCTGGGGCCCGGGGGACTTCCCGGCT TCTACACACAGCCCACCTTCTTGGAAGTGCTGTCCGATCCGCAGAGCGTCCACTTGCAGCCACTAGGCAG AGCGTCGGGGCCGCTGGACACCAGCCAGACGGCCAGCTCCGATTCCGAAGATGTTTCCTCCAGTGACCGA AAAATGTCCAAATCGGCTTTAAACCAGACCAAGAAGCGTAAGAAGCGGCGACACAGGACAATCTTTACTT CCTACCAGCTAGAGGAGCTGGAGAAAGCATTCAATGAAGCCCACTACCCAGATGTCTACGCCCGGGAGAT GCTGGCCATGAAAACGGAGCTCCCAGAAGACAGGATACAGGTGTGGTTCCAGAACCGCAGAGCCAAGTGG AGGAAGAGGGAGAAGTGCTGGGGCCGGAGCAGCGTCATGGCTGAGTATGGTCTCTATGGAGCCATGGTGA GGCACTCGATCCCCCTGCCAGAGTCTATCCTCAAGTCTGCCAAGGATGGCATAATGGATTCCTGTGCCCC ATGGCTACTGGGGATGCACAAAAAGTCGCTGGAGGCAGCAGCTGAGTCGGGAAGGAAGCCCGAGGTGGAA CGCCAGGCCCTGCCCAAGCTCGACAAGATGGAGCAGGAGGAACGGGCCCCAGAGGCCCAGGCAGCCATCT CCCAGGAAGAACTGAGGGAGAACAGCATTGCCGCCCTCCGAGCCAAAGCTCAGGAACACAGCACCAAGGT TCTGGGGACTGTATCTGGGCCTGACAGCCTGGCCAGAAATGCTGAGAAGCCAGAAGAAGAGGACGCCACG GAGGAAGACAGGCCAGCTGAGAAGCTCAGCCCACCGCAGCTGGAGGACATGGCTTAGGGCAAGGGCTGCT GGCCTCGGAACTGCTCTTCTCCAGCCCTGCAGCCCTGCGGTGTGGCGAGTTCTCTCTGAGGCAAGGCCCA TGTCTGGCTTCTGCCACACCTCCCCACACCTCAGGCCCGCAATTACCCCCACGACTCTAGAAACAGACCA ATTCTGTCCGTAGCTTTGGACCATGCTGAGACATCCTGGACATACTCCCGATCCAGCGTCCTCAGCCTCA GCCCAGCTTCCCTGCCTCCAAAGCCTTCTTGCTACCCCGACATCGAGGTCCTGCTACGTCATCCCTGAGA GACTCTTGTCCAAGCCTATCCCTTATTCTCTGCTCTCTAAGCCTTCCTCCCCAGCTTAGCCCCCTCATGG GCGGCTGCGCCCACTGCTGTAAACCCAGCCCACAGCCGGGCAGAGCTGTGATTGGATTGAGGCCCTGCCT CGGTGTCCCGAGCACCCCAGCACTTTGCAGGCAGGCTTTTCCCTCTTCACCTGTTCCTTCTCGCAGACCT GGACCCTGGCTGGCTCCTGCTGTGTGAATGCACAGCCGGGTCACGTGGGTCCTGCTGTGAGGATGCACTG CTCAGCTGGTCCACGGTGTAACCTCTGCATTCTCTGCCCTGCCTGCTGATGGGCAGATTCAAGTCTAAAC AGGGCAACTCTCAGATTGAGTCCTTCCCCAAACCTGTCTCACGCCGTGCTGTAGATCATCCTCAGGCCTT CTCCGCACCGAGCCTGCCCTACATGTTCCTCCTTTGCAGAGGTGGGCCACACCACCTTGTCAGTGTCTAG GCCCTGCAGCCTGAGGGCGGGGCGCTTCTCGGCAATGCTGTGGCTTGCTTTACTGCTGGTGAGCCCTCAC CCAGAGCTTTTGCACCCCAGGGCCTGGGTGTCTGAAGCATTTTGACCCTTCTGCCTATGACTTTGACTTG CTAGTTAAGGCTTCTTTCTAGAGCTTCCTGTAGCCCAGTGGCTCTCAAGCTTCCTAATGCTATGACCTTT AATACAGTTCCTTGTGTTGTGGTGACCACCCCCCCCCAACCTTTAAATGTATTTCATTGCTACTTCATAA CTGTAATTTTGCTACTGTTATGAATCAAAATGTAAATATTTGATATGTGGAATATCTGATTATGTGACTT CCTCCAAAGGGGCCATGGGGGTCATGACCCACAGGTTGAGAACTGCTGTTGTAGCCCATTGGTTCACATG TGCCTGCTTTAGAGAAAATGGGTGCAGCTTAAAAGCCCCGGACATGGCCTCACTTTGACCTAGGCAGTCC TAAAAGGCCAAGCCAAAATCAGACCCACGCTATCTATCTATACACACACGCACACGCACACATGCACGCA TGCACGCACGCTGTATAACATGAAAGGTACGGGGAAAGCTCAAAGAACAAGGCAGGTAGCTAGTAAAAAA ACCAGACTAAGCTACAGACGCTCATAGCTCCGTGCCAGCTGTATGCCCAGAAAGGTGTCCATAGATAAGG AGCCATGTTGGACTGAATGCCTTCTGCCTGCAAAGGAGTGGCACTGTCCATACTGATGGCATGTCTTCAG ATGTCCCTGTCTGCAGATGTCTCTGTCTGCAGATGCCCCTTCCACCTGGCTTTATACATGTACTAGGGGT GCAGAGCCTTCTGGGGTCCTCTGCTCCTGTATTCCCAGGCCAACCACAAGACCCCCTTTATCCTGTGAAG TTACCCCACCTACCACTTCACAAAAAAGGCCCGGGCAGTGAGTTGTGATTTTAAATCTGTTTTGACAGTT GATTTCTTCTTCGTTCTCTTCATAGACTTCTCCCGCTTCTGAGTTCTTATCTTCTCCCGGGTTGAGATGT TGACTATAGTCCTGGTGTGTAAATAGTGTACATAATGTGAGAAGGAAGAAGCTGGACACTGAGGTGTTCG AAACACTGAATTGTCATAACTTTTAGGTATTCAAAGCATAATTTCTGTGCTGTTTTCTGTAAAGACACAA GCAAGAATAAAATTCATGCAAAGACTGCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4480964 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4480964 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16373 same embryo
	EMAGE:16371 same embryo
	EMAGE:16372 same embryo
	EurExpress:euxassay_017155 same experiment
	MGI:4829179 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source