EMAGE mouse gene expression spatial database

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EMAGE:16371

Capza2 capping protein (actin filament) muscle Z-line, alpha 2 ( MGI:106222)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017154. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017154_01	euxassay_017154_02	euxassay_017154_03	euxassay_017154_04

euxassay_017154_05	euxassay_017154_06	euxassay_017154_07	euxassay_017154_08	euxassay_017154_09

euxassay_017154_10	euxassay_017154_11	euxassay_017154_12	euxassay_017154_13	euxassay_017154_14

euxassay_017154_15	euxassay_017154_16	euxassay_017154_17	euxassay_017154_18	euxassay_017154_19

euxassay_017154_20	euxassay_017154_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16371_wholemount_strong.wlz

16371_wholemount_moderate.wlz

16371_wholemount_weak.wlz

16371_wholemount_possible.wlz

16371_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16371_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9611
Entity Detected:	Capza2, capping protein (actin filament) muscle Z-line, alpha 2 ( MGI:106222)
Sequence:	sense strand is shown >T9611 CCACGGTCTGTCGCCAGAAGGAAGATGGCGGATCTGGAGGAGCAGTTGTCCGATGAGGAGAAGGTTCGTA TAGCAGCAAAATTCATCATTCACGCCCCTCCTGGAGAATTTAATGAGGTTTTTAATGATGTTCGATTATT GCTTAATAATGATAATCTTCTCAGGGAAGGAGCCGCACATGCATTTGCACAGTATAACCTGGACCAGTTT ACACCAGTAAAAATTGAAGGCTATGAAGATCAGGTTTTGATAACAGAACATGGAGACTTGGGAAATGGAA AATTTTTGGATCCAAAAAACAGAATCTGTTTCAAATTTGATCATTTAAGAAAGGAAGCAACTGATCCAAG GCCCTACGAAGCAGAAAATGCCATTGAATCCTGGAGAACTTCAGTAGAAACTGCACTGCGAGCTTATGTA AAAGAGCATTATCCGAATGGGGTCTGCACTGTGTATGGCAAAAAAGTAGATGGGCAGCAAACCATTATTG CATGCATAGAAAGCCATCAGTTTCAAGCAAAAAACTTTTGGAATGGTCGTTGGAGGTCAGAGTGGAAGTT TACAGTCACTCCTTCCACCACACAGGTGGTTGGCATTTTGAAAATTCAGGTTCATTATTATGAAGATGGT AATGTTCAGCTAGTGAGTCATAAAGATATACAAGATTCCCTCACAGTATCTAATGAGGTGCAAACAGCAA AAGAATTTATAAAGATTGTAGAAGCTGCAGAAAATGAATACCAGACTGCCATCAGTGAGAATTATCAGAC GATGTCGGACACTACCTTCAAAGCCTTACGTCGACAGTTGCCAGTGACACGTACTAAGATCGACTGGAAC AAGATCCTTAGCTACAAGATTGGCAAAGAGATGCAGAATGCATAAATGAAAATTGCATGAGCAGATTGTT TTAGTGTCTTCGCGTTACAAAAATTATTGCAAAAGTATTCTGAGCTGACAGCTGCCCAGTGAGGTGGGCT GTTGCATCTAAGATCACTGTAATTAAGTAGTGTGGTTAGAGCACAGAGCTTAGCTAATAACCTGTTCCAT TTCCTTTGTTCAGAGGGTTGGACATGAGTTTAGATAAGTATCTTACTCTGTTCCTCCTTTCGAAGTCTCT CTCTCTCTCTCTTTCTTTCTCTCTCTCTCTGTTCCTTCTCCTACAATGAAGAGGTGATCCCTCTGGGTCA TGTCTAAAGGGTTTGAAATATCTCAAAAGTACCTTCTTTACTGTAACCCTGAAGTTGTCTTTGGCTTGTG AAAGGGGCAGCTTTTGATGTTAGCCCAGGTAGTTTTAGTGGGGTCAGTCAGTAATGGGCACTCTAGTTAA GGTGGTTGATGGACTTAGCCATGTATGCTGCTAAAGAAATTGTCTACCTTTTCCTTTCACCTCTTCCAGC ATGGAAGATGGAGATTAGAGGGAGAGCATTTTCTAACGAGTTACGTTAAACCTTCTCAGCAAGGTTATTT AGCTAGCTTAGTGTCTAACTAAACTTTTTTAAAACAAGGCCAAGGTCTGTTGCTGTTTTGAGATTCTGAA ATTAAATGAGAATACTTATTTCAGAAATGCATTTAATGCTTTTTTCTTGTGACAGTTATGCAAATTAGCT TGAATTCCATATGTTCCTGAGTTATTTTTATCACAAAGCCACAAGTGTATTATAAGGCAGATTGTAATAT ATATATAATCCTGAACTCATGAACATGTCTCAGTTGATTCTCCCTCCCCTTGGATTGGAAACTGAATATC AGTGTGACATTAAAATGGCAACATTTGGTATTTATTTGAGTAAAAAAAAAATCACCAGTGAATCATACAC TATTTTAAAATACTTTCTTTGTATATTGTCATTCTTAACAAGTTGTAAATTAGAAAATTTGGGAGTCCAC ATGGTATGCAACTGCATCTTTTGTTCTCCATTTTCCTATGCATCATAAGCATGTTTGTAATACTTTAAAA ATATATCTACTGCCACCACAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30626416 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30626416 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16373 same embryo
	EMAGE:16370 same embryo
	EMAGE:16372 same embryo
	EurExpress:euxassay_017154 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source