EMAGE mouse gene expression spatial database

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EMAGE:16380

Itih4 inter alpha-trypsin inhibitor, heavy chain 4 ( MGI:109536)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017184. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017184_01	euxassay_017184_02	euxassay_017184_03	euxassay_017184_04

euxassay_017184_05	euxassay_017184_06	euxassay_017184_07	euxassay_017184_08	euxassay_017184_09

euxassay_017184_10	euxassay_017184_11	euxassay_017184_12	euxassay_017184_13	euxassay_017184_14

euxassay_017184_15	euxassay_017184_16	euxassay_017184_17	euxassay_017184_18	euxassay_017184_19

euxassay_017184_20	euxassay_017184_21	euxassay_017184_22	euxassay_017184_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16380_wholemount_strong.wlz

16380_wholemount_moderate.wlz

16380_wholemount_weak.wlz

16380_wholemount_possible.wlz

16380_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16380_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4965
Entity Detected:	Itih4, inter alpha-trypsin inhibitor, heavy chain 4 ( MGI:109536)
Sequence:	sense strand is shown >T4965 GTCGACCCACGCGTCCGGGCAGATGAAGAGCCCTGCCCCTGCCCACATGTGGAACCTTGTGCTGTTCTTG CCTTCACTGTTGGCTGTGCTTCCGACCACTACTGCCGAGAAGAATGGCATCGATATCTACAGCCTCACGG TGGACTCCCGGGTCTCTTCCCGATTTGCCCATACTGTTGTCACCAGCCGGGTGGTCAACAGAGCCGATGC TGTTCAAGAAGCGACCTTCCAAGTAGAGCTACCCAGGAAAGCCTTCATCACCAACTTCTCCATGATCATC GATGGCGTGACCTACCCAGGGGTTGTCAAAGAGAAGGCCGAAGCCCAGAAACAATACAGTGCCGCCGTGG GCAGGGGAGAGAGTGCTGGCATCGTCAAGACCACTGGGAGGCAGACAGAGAAGTTTGAAGTGTCAGTCAA CGTGGCCCCTGGTTCCAAGATTACCTTCGAACTCATATACCAGGAACTGCTCCAAAGGCGACTGGGAATG TATGAGCTACTCCTCAAAGTGAGGCCTCAGCAGCTGGTGAAGCACCTTCAGATGGACATCTACATCTTTG AGCCTCAGGGTATTAGCATCCTGGAGACAGAGAGCACCTTCATGACCCCGGAGCTGGCAAATGCCCTTAC CACTTCACAGAACAAGACCAAGGCTCATATCCGGTTCAAGCCGACGCTCTCCCAGCAACAGAAGTCTCAG AGTGAGCAGGACACGGTGCTGAATGGGGACTTCATCGTCCGCTATGATGTCAACCGGTCTGACTCTGGGG GCTCCATTCAGATTGAGGAAGGCTACTTTGTGCACCACTTTGCTCCAGAGAACCTTCCTACAATGTCCAA GAATGTGATCTTTGTCATTGATAAAAGCGGATCTATGTCAGGCAAGAAAATCCAGCAGACCCGAGAAGCC CTAGTCAAGATCTTGAAAGACCTCAGCCCCCAAGACCAGTTCAACCTCATTGAGTTCAGTGGGGAAGCAA ACCAATGGAAGCAGTCACTGGTGCAAGCGACAGAAGAGAATTTGAACAAGGCTGTAAACTATGCTTCCAG GATCCGGGCTCACGGAGGGACCAACATCAATAATGCAGTGCTGTTGGCTGTGGAGCTGCTGGACAGAAGC AACCAAGCTGAGCTACTGCCCTCGAAGAGCGTCTCCCTTATCATCCTGCTCACGGACGGTGACCCCACTG TGGGAGAAACCAACCCCACGATTATCCAGAACAACGTGCGGGAAGCCATCAATGGGCAGTATAGCCTCTT CTGCCTGGGGTTCGGCTTTGATGTGAACTATCCTTTCCTGGAGAAGATGGCACTGGACAATGGTGGCCTG GCCAGGCGCATCTATGAGGATTCAGACTCTGCACTGCAGCTTCAGGATTTCTACCACGAAGTAGCCAATC CACTGCTCTCATCAGTGGCCTTCGAATACCCCAGTGATGCTGTGGAGGAAGTCACTCGGTACAAGTTCCA ACACCACTTTAAGGGCTCAGAGATGGTGGTGGCTGGGAAGCTCCAGGACCAGGGTCCTGATGTCCTCTTA GCCAAAGTCAGTGGGCAGATGCACATGCAGAACATCACTTTCCAAACGGAGGCCAGCGTAGCCCAACAAG AGAAGGAGTTTAAGAGCCCCAAGTACATCTTTCACAACTTTATGGAGAGACTGTGGGCACTGCTGACTAT ACAGCAACAGCTGGAGCAGAGGATTTCAGCGTCAGGTGCCGAATTAGAGGCCCTCGAGGCCCAAGTTCTG AACTTGTCACTCAAGTACAATTTTGTCACCCCTCTCACGCACATGGTGGTCACCAAACCTGAAGGTCAAG AACAATTCCAAGTTGCTGAGAAGCCTGTGGAAGTCGGTGATGGCATGCAGAGACTCCCCTTAGCAGCTCA AGCCCACCCCTTCAGGCCTCCTGTCAGAGGATCTAAACTGATGACCGTGCTGAAAGGAAGCAGGTCCCAG ATACCCAGACGCGGTGATGCCGTTAGGGCATCTAGGCAATACATTCCTCCCGGATTCCCCGGACCTCCTG GACCTCCCGGATTTCCTGCACCCCCTGGACCTCCTGGATTTCCTGCACCCCCTGGACCTCCTCTTGCTTC TGGCTCTGACTTCAGCCTTCAGCCTTCCTATGAAAGGATGCTAAGCCTGCCCTCCGTTGCAGCACAATAT CCTGCTGACCCACATCTGGTTGTGACGGAAAAAAGTAAAGAAAGCACCATACCAGAGGAATCCCCAAACC CAGACCACCCCCAGGTTCCTACTATTACCTTGCCGCTTCCGGGATCCAGTGTGGACCAGCTCTGTGTGGA TATCTTACATTCTGAGAAGCCCATGAAGCTGTTCGTAGACCCCAGTCAGGGTCTGGAGGTGACTGGTAAG TATGAGAATACTGGGTTCTCGTGGCTCGAAGTGACCATCCAGAAGCCTCACCTGCAGGTCCATGCAACCC CTGAACGACTGGTGGTGACACGAGGCAGAAAAAACACTGAATACAAGTGGAAGAAGACGCTGTTCTCTGT GTTACCTGGCTTGAAGATGACCATGAATATGATGGGACTCCTACAGCTCAGTGGCCCAGACAAAGTCACC ATCGGCCTCCTGTCCCTGGATGACCCTCAGAGAGGACTAATGCTGCTTTTGAATGACACCCAGCACTTCT CCAACAACGTTAAAGGGGAGCTTGGTCAGTTTTACCGGGACATCGTCTGGGAGCCACCCGTCGAGCCAGA TAATACAAAACGGACAGTCAAAGTTCAAGGAGTTGACTACCTGGCTACCAGAGAGCTCAAGTTGAGTTAC CAAGAAGGGTTCCCAGGAGCAGAGATTTCCTGCTGGACAGTGGAGATATAGAACTGTTAGGAGCGCCGCT CCCTGCCATGTTGTCCTCGTACGCAGGCAGATGACACCTTATGCCAACAGGGACGCCTGTGAGGCCGAGA CCTTGATGGGAAGAGGATGCTCCCTTGTTACAAATAAAGAAGGGCAGTGTGAACCCAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136326 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136326 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16381 same embryo
	EurExpress:euxassay_017184 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source