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EMAGE:16419

Mir199b microRNA 199b ( MGI:2676865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419
"Pseudo-wholemount" of euxassay_019074. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019074_01 euxassay_019074_02 euxassay_019074_03 euxassay_019074_04
EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419
euxassay_019074_05 euxassay_019074_06 euxassay_019074_07 euxassay_019074_08 euxassay_019074_09
EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419
euxassay_019074_10 euxassay_019074_11 euxassay_019074_12 euxassay_019074_13 euxassay_019074_14
EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419 EMAGE:16419
euxassay_019074_15 euxassay_019074_16 euxassay_019074_17 euxassay_019074_18 euxassay_019074_19
EMAGE:16419 EMAGE:16419
euxassay_019074_20 euxassay_019074_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16419Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16419_wholemount_strong.wlz
16419_wholemount_moderate.wlz
16419_wholemount_weak.wlz
16419_wholemount_possible.wlz
16419_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16419_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
mesenchyme
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70316
Entity Detected:Mir199b, microRNA 199b ( MGI:2676865)
Sequence:sense strand is shown

>T70316
ACAGTAGTCTGCACATTGGTTA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-199b was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16420 same assay
 EMAGE:16421 same assay
 EMAGE:16417 same embryo
 EurExpress:euxassay_019074 same experiment
 EMAGE:16416 same embryo
 MGI:4826237 same experiment
 MGI:4826238 same experiment
 MGI:4826236 same experiment
 EMAGE:16418 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS