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EMAGE:16520

Cbx4 chromobox homolog 4 (Drosophila Pc class) ( MGI:1195985)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520
"Pseudo-wholemount" of euxassay_016694. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016694_01 euxassay_016694_02 euxassay_016694_03 euxassay_016694_04
EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520
euxassay_016694_05 euxassay_016694_06 euxassay_016694_07 euxassay_016694_08 euxassay_016694_09
EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520
euxassay_016694_10 euxassay_016694_11 euxassay_016694_12 euxassay_016694_13 euxassay_016694_14
EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520 EMAGE:16520
euxassay_016694_15 euxassay_016694_16 euxassay_016694_17 euxassay_016694_18 euxassay_016694_19
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euxassay_016694_20 euxassay_016694_21 euxassay_016694_22 euxassay_016694_23 euxassay_016694_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16520Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16520_wholemount_strong.wlz
16520_wholemount_moderate.wlz
16520_wholemount_weak.wlz
16520_wholemount_possible.wlz
16520_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16520_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45053
Entity Detected:Cbx4, chromobox homolog 4 (Drosophila Pc class) ( MGI:1195985)
Sequence:sense strand is shown

>T45053
ATGAGCAAGTACATGGAGAACGGCATGCAGGCAGTGAAAATCAAGTCGGGCGAGGCCGCCGAGGGCGAGG
CGCGCTCCCCCAGCCACAAGAAACGTGCTGCCGAGGAGCGCCATCCCCAGGGCGACAGGACTTTTAAAAA
GGCAGCCGGTGCCTCTGAGGAGAAGAAAGCCGAAGTGCCCTGCAAACGCAGGGAGGAGGAGGCTCTGGTG
TCGGGGGACGCGCAGCCCCAGGACCTAGGGTCTCGAAAGCTCTCCCCGACCAAGGAGGCCTTTGGTGAGC
AACCGCTACAGCTCACCACCAAGCCAGACCTGCTGGCCTGGGACCCAGCCAGGAGCTCGCACCCGCCCGC
CCACCACCACCATCACCACCACCATCACCACCACCACCATACGGTGGGCCTGAACCTCTCCCATGCGCGC
AAGCGCTGCCTCTCCGAGACCCATGGGGAGCGTGAGCCCTGTAAGAAGCGGTTGACCGCCCGCAGCATCA
GCACGCCGACCTGCCTGGGGGGCAGCCCGGTCTCCGAGCACCCTGCCAACGTATCCCCCACCGCGGCCTC
TCTGCCGCAGCCCGAGGTCATCCTTTTAGACTCAGACCTGGATGAACCCATAGACTTGCGCTGTGTCAAG
ATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 166598. Forward Primer - name:166598_F_exon_Cbx4, sequence:ATGAGCAAGTACATGGAGAACG; Reverse Primer - name:166598_N_SP6_exon_Cbx4, sequence:CATCTTGACACAGCGCAAGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16519 same embryo
 EMAGE:16521 same embryo
 EMAGE:16517 same embryo
 EMAGE:16518 same embryo
 EurExpress:euxassay_016694 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS