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EMAGE:16527

Ski ski sarcoma viral oncogene homolog (avian) ( MGI:98310)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16527
euxassay_016716_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16527Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16527_wholemount_strong.wlz
16527_wholemount_moderate.wlz
16527_wholemount_weak.wlz
16527_wholemount_possible.wlz
16527_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16527_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45179
Entity Detected:Ski, ski sarcoma viral oncogene homolog (avian) ( MGI:98310)
Sequence:sense strand is shown

>T45179
AGACCATCTCTTGTTTCGTGGTGGGAGGTGAGAAGCGTCTGTGCCTGCCGCAGATCCTCAACTCGGTGCT
GCGCGACTTCTCACTGCAGCAGATCAACTCCGTGTGCGATGAGCTGCACATCTACTGCTCGCGCTGCACC
GCCGACCAGCTGGAGATCCTCAAAGTCATGGGCATCCTGCCCTTCTCCGCGCCCTCCTGCGGGCTCATCA
CCAAGACGGACGCCGAGCGCCTGTGCAACGCGCTGCTCTACGGCGGCGCCTACCCGCCGCCTTGCAAGAA
GGAGTTGGCGGCCAGCCTGGCGCTGGGCCTGGAGCTCAGCGAACGCAGCGTCCGCGTGTACCACGAGTGC
TTCGGCAAGTGCAAAGGGCTGCTGGTGCCCGAGCTGTACAGCAGTCCCAGCGCTGCCTGCATCCAGTGCC
TCGACTGCCGCCTCATGTACCCGCCGCACAAGTTCGTTGTACACTCGCACAAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 164638. Forward Primer - name:164638_F_Ski, sequence:AGACCATCTCTTGTTTCGTGGT; Reverse Primer - name:164638_R_SP6_Ski, sequence:CCTTGTGCGAGTGTACAACGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16530 same embryo
 EMAGE:16529 same embryo
 EMAGE:16528 same embryo
 EMAGE:16532 same embryo
 EMAGE:16531 same embryo
 EurExpress:euxassay_016716 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS