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EMAGE:16531

Tbx21 T-box 21 ( MGI:1888984)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531
"Pseudo-wholemount" of euxassay_016718. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016718_01 euxassay_016718_02 euxassay_016718_03 euxassay_016718_04
EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531
euxassay_016718_05 euxassay_016718_06 euxassay_016718_07 euxassay_016718_08 euxassay_016718_09
EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531
euxassay_016718_10 euxassay_016718_11 euxassay_016718_12 euxassay_016718_13 euxassay_016718_14
EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531
euxassay_016718_15 euxassay_016718_16 euxassay_016718_17 euxassay_016718_18 euxassay_016718_19
EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531 EMAGE:16531
euxassay_016718_20 euxassay_016718_21 euxassay_016718_22 euxassay_016718_23 euxassay_016718_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16531Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16531_wholemount_strong.wlz
16531_wholemount_moderate.wlz
16531_wholemount_weak.wlz
16531_wholemount_possible.wlz
16531_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16531_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45499
Entity Detected:Tbx21, T-box 21 ( MGI:1888984)
Sequence:sense strand is shown

>T45499
GGACTAGGCGAAGGAGACACTAAGAGGAGGAGGATATCCCCCTATCCTTCCAGTGGCGACAGCTCCTCTC
CCGCTGGGGCCCCTTCTCCTTTTGATAAGGAAACCGAAGGCCAGTTTTATAATTATTTTCCCAACTGAGA
AAATGCCGCTGAATTGGAAGGTGCCCACTAACTTAGAAAACAGACGCGGGGCTGAGAGCCCCGAGCTCTT
CCCCATCCCTTCCCTGTATAGTGATTGGTTGGAGAGGAAGCGGGGCAAGAAGGATTCTGGGGTTTACTTC
TTGTTTCCTGGCCCACAAGGAAATACGACAGGAGTGTCCCCTGCCCCTTTCTCTGCCCGAACTACAGTCA
CGAACCTGGTGCTGCTTCTGACCCCATGGTTCCATGGAGAACGGAGAATGGACTCCAGAGAGTTTTGGAC
CCAGAGGGACTTCATGGCTTTCTGCGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 172712. Forward Primer - name:172712_F_Tbx21, sequence:GGACTAGGCGAAGGAGACACTA; Reverse Primer - name:172712_R_SP6_Tbx21, sequence:CTCGCAGAAAGCCATGAAGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16530 same embryo
 EMAGE:16529 same embryo
 EMAGE:16528 same embryo
 EMAGE:16532 same embryo
 EMAGE:16527 same embryo
 EurExpress:euxassay_016718 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS