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EMAGE:16542

Kcnq1ot1 KCNQ1 overlapping transcript 1 ( MGI:1926855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542
"Pseudo-wholemount" of euxassay_016726. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016726_01 euxassay_016726_02 euxassay_016726_03 euxassay_016726_04
EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542
euxassay_016726_05 euxassay_016726_06 euxassay_016726_07 euxassay_016726_08 euxassay_016726_09
EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542
euxassay_016726_10 euxassay_016726_11 euxassay_016726_12 euxassay_016726_13 euxassay_016726_14
EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542
euxassay_016726_15 euxassay_016726_16 euxassay_016726_17 euxassay_016726_18 euxassay_016726_19
EMAGE:16542 EMAGE:16542 EMAGE:16542 EMAGE:16542
euxassay_016726_20 euxassay_016726_21 euxassay_016726_22 euxassay_016726_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16542Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16542_wholemount_strong.wlz
16542_wholemount_moderate.wlz
16542_wholemount_weak.wlz
16542_wholemount_possible.wlz
16542_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16542_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38978
Entity Detected:Kcnq1ot1, KCNQ1 overlapping transcript 1 ( MGI:1926855)
Sequence:sense strand is shown

>T38978
CCATTCTCCTTGTACCCTTGAGGATGAAGCTGAACACAAACATGGCTCCATCTACCACCATGGAGAGAGG
GGGGGCGGGACATGACATAAAGTCTATCCATAAGGGAAAAGATGTCTGATTATACTGAAACACAAGTGTA
CCTGAGAATGATAGCAGAATATTCTAGTTTCATTTCTGTGGCTGTGACAAGTACCCTGGCCAATAAAACA
ACCTAGGGGTGGTGGGGGAAGGGGTCAATGTAGCTAACAATTCCAAGGGTGGGGTGGTTTAATGTGGCTA
ACAATTTCAGCACAGTTCATTATTTCAGGGACGTCAAGACAGGAACTCAAATGGCAAGTCACATGTGCAC
AGTCAGGAGTAGAGAGAAAAATACAACCTTGCTTGCTTGCTTCTCAGTTAGGGTTGTACTGGTTTATCTA
GTTCAGGGCCCCAAGCCTAGGGAATGGTGGCACTCATCATGGCCCTTGATAATTCCTTAAGTGAGACTTT
CTTCTAAGAGGATTCTAGGATGTGACACGCTGGCACTTAAAACTAACTGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 265087. Forward Primer - name:265087_F_cDNA_Kcnq1ot1, sequence:CCATTCTCCTTGTACCCTTGAG; Reverse Primer - name:265087_N_SP6_cDNA_Kcnq1ot1, sequence:GCCAGTTAGTTTTAAGTGCCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16543 same embryo
 EMAGE:16541 same embryo
 EMAGE:16540 same embryo
 EMAGE:16539 same embryo
 EurExpress:euxassay_016726 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS