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EMAGE:16579

Fndc3b fibronectin type III domain containing 3B ( MGI:1919257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579
"Pseudo-wholemount" of euxassay_016765. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016765_01 euxassay_016765_02 euxassay_016765_03 euxassay_016765_04
EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579
euxassay_016765_05 euxassay_016765_06 euxassay_016765_07 euxassay_016765_08 euxassay_016765_09
EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579
euxassay_016765_10 euxassay_016765_11 euxassay_016765_12 euxassay_016765_13 euxassay_016765_14
EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579 EMAGE:16579
euxassay_016765_15 euxassay_016765_16 euxassay_016765_17 euxassay_016765_18 euxassay_016765_19
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euxassay_016765_20 euxassay_016765_21 euxassay_016765_22 euxassay_016765_23 euxassay_016765_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16579Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16579_wholemount_strong.wlz
16579_wholemount_moderate.wlz
16579_wholemount_weak.wlz
16579_wholemount_possible.wlz
16579_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16579_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63193
Entity Detected:Fndc3b, fibronectin type III domain containing 3B ( MGI:1919257)
Sequence:sense strand is shown

>T63193
CTCCTTCCAGAAACGACATACCGGATCAGAATTCAGGCTATCAATGAAATTGGAGTTGGACCATTTAGTC
AGTTCATTAAAGCAAAAACTCGGCCATTACCGCCTTCGCCTCCTAGGCTTGAGTGTGCTGCGTCTGGTCC
TCAGAGCCTGAAGCTCAAGTGGGGAGACAGTAACTCCAAGACACATGCTGCTGGTGACATGGTGTACACA
CTACAGCTGGAAGACAGGAACAAGAGGTTTATCTCAATCTACCGAGGACCCAGCCACACCTACAAGGTCC
AGAGACTGACAGAGTTTACCTGCTACTCCTTCAGGATCCAGGCAATGAGCGAGGCAGGGGAGGGGCCTTA
CTCAGAAACCTACACCTTCAGCACAACCAAAAGCGTGCCTCCCACCCTCAAAGCACCTCGAGTGACGCAG
TTAGAAGGGAATTCCTGTGAAATCTTCTGGGAGACGGTACCACCGATGAGAGGCGACCCTGTGAGCTACG
TTCTACAGGTGCTGGTTGGAAGAGACTCTGAGTACAAGCAGGTGTACAAGGGAGAAGAAGCCACATTCCA
AATCTCAGGCCTCCAGAGCAACACAGATTACAGGTTCCGCGTGTGTGCCTGCCGCCGCTGTGTGGACACG
TCTCAGGAGCTCAGTGGCGCGTTCAGCCCCTCTGCGGCTTTCATGTTACA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 148696. Forward Primer - name:148696_F_cDNA_1600019O04Rik, sequence:CTCCTTCCAGAAACGACATACC; Reverse Primer - name:148696_N_SP6_cDNA_1600019O04Rik, sequence:TGTAACATGAAAGCCGCAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16578 same embryo
 EMAGE:16580 same embryo
 EMAGE:16582 same embryo
 EMAGE:16581 same embryo
 EMAGE:16577 same embryo
 EurExpress:euxassay_016765 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS