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EMAGE:16581

Ankrd12 ankyrin repeat domain 12 ( MGI:1914357)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581
"Pseudo-wholemount" of euxassay_016760. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016760_01 euxassay_016760_02 euxassay_016760_03 euxassay_016760_04
EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581
euxassay_016760_05 euxassay_016760_06 euxassay_016760_07 euxassay_016760_08 euxassay_016760_09
EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581
euxassay_016760_10 euxassay_016760_11 euxassay_016760_12 euxassay_016760_13 euxassay_016760_14
EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581
euxassay_016760_15 euxassay_016760_16 euxassay_016760_17 euxassay_016760_18 euxassay_016760_19
EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581 EMAGE:16581
euxassay_016760_20 euxassay_016760_21 euxassay_016760_22 euxassay_016760_23 euxassay_016760_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16581Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16581_wholemount_strong.wlz
16581_wholemount_moderate.wlz
16581_wholemount_weak.wlz
16581_wholemount_possible.wlz
16581_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16581_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63493
Entity Detected:Ankrd12, ankyrin repeat domain 12 ( MGI:1914357)
Sequence:sense strand is shown

>T63493
GACATCCACAGCCATTGAGTTACTGTGTCTTCTCAGCTGGCCTAGGGTCAGGGTAACTTTGTCTTTAACA
GTTCTTTTTTACTAGTTTGAAATTGTATCCTTTATTGTATTTAATCAGCACTATTCATAACATCAGTTTT
TAATTTTCTCATTGTGTGCGTGACATTTTATTATTGTATCCAACATTGGATTACAAGGTCTGTAAGAGCA
AGAATCCTCTGTTTCCTGGGCGGTAAGTAAGTGCTTGTTAAATAAGACCTGTGATTTGAAATTGAAGTAT
TTGTAAGTGGGATCCTATGTAAATTTCTTTAAAAATAAAATAAATCCTTAACTTTTAAGATAAAATTTAA
ACATTTTTTAGGAAATTATTTATTTTTATGAGAGTGTGTGTGCGTGTGTGTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 111217. Forward Primer - name:111217_F_cDNA_LOC432413, sequence:GACATCCACAGCCATTGAGTTA; Reverse Primer - name:111217_N_SP6_cDNA_LOC432413, sequence:ACACACACACGCACACACACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16578 same embryo
 EMAGE:16580 same embryo
 EMAGE:16582 same embryo
 EMAGE:16577 same embryo
 EMAGE:16579 same embryo
 EurExpress:euxassay_016760 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS