Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:16589

Rfx5 regulatory factor X, 5 (influences HLA class II expression) ( MGI:1858421)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589
"Pseudo-wholemount" of euxassay_016784. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016784_01 euxassay_016784_02 euxassay_016784_03 euxassay_016784_04
EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589
euxassay_016784_05 euxassay_016784_06 euxassay_016784_07 euxassay_016784_08 euxassay_016784_09
EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589
euxassay_016784_10 euxassay_016784_11 euxassay_016784_12 euxassay_016784_13 euxassay_016784_14
EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589
euxassay_016784_15 euxassay_016784_16 euxassay_016784_17 euxassay_016784_18 euxassay_016784_19
EMAGE:16589 EMAGE:16589 EMAGE:16589 EMAGE:16589
euxassay_016784_20 euxassay_016784_21 euxassay_016784_22 euxassay_016784_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16589Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16589_wholemount_strong.wlz
16589_wholemount_moderate.wlz
16589_wholemount_weak.wlz
16589_wholemount_possible.wlz
16589_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16589_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63088
Entity Detected:Rfx5, regulatory factor X, 5 (influences HLA class II expression) ( MGI:1858421)
Sequence:sense strand is shown

>T63088
GTGATCCAAGACCTCATGACAAGGGTATCAAAAGGACAGCTGAAGTGCCTCTGAGTGAGGCCAGTGGGCA
GGACCCACCAGTTAAAGAAATGAAGCACGAAACACAGGATACTACAGTAAGTGAAGCAAAAAGGAAGCGA
GGACGCCCTCGAAAGAAGCCAGGAGGGAGTGGGGAAAGAAACGCTACTCCTGAGAAGTCTGCAGCTATTG
TGAACTCTCCCCGATCCCCAAGGCTACTCTGGGAGACATGGGGCTCAAAAAGAGAAAACAACTTCATTGG
GAGACCAGAAGGACCAGGGCCAGGGGGTGAGGCTGAGCGGGAAACAGTGCTTGTGCAAGGTCAGCAAGAT
GGTGCTGTTTCCAAAGGAGAAAGGAGCTTGAGTTCCCAGGAAGCCAAAGAAGCAGAAGATAAAATTCCTC
CTGTCACTTCAAAAGTGAGTGTTATCAAGGGCAGAATTCAAAAAGAGGCTCTTCAGTTGGTAAAGGGAGA
GGCTGATGCTGCAACACAGGGCAACAAAGGGTTAAAGGGTCGTGTACTTCAAAGTTCCCTAACCCCTGAG
CATAAAGACCCCAAAGCAACACCCCCATGATAGACAGATAGGTCTGTGGGGGAAGAGTGGCTATGTTCAT
GTGAGCTCTGCCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 153792. Forward Primer - name:153792_F_cDNA_TC1514152, sequence:GTGATCCAAGACCTCATGACAA; Reverse Primer - name:153792_N_SP6_cDNA_TC1514152, sequence:CAGGCAGAGCTCACATGAACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16587 same embryo
 EMAGE:16588 same embryo
 EMAGE:16585 same embryo
 EMAGE:16590 same embryo
 EMAGE:16586 same embryo
 EurExpress:euxassay_016784 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS