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EMAGE:16607

Gnaq guanine nucleotide binding protein, alpha q polypeptide ( MGI:95776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607
"Pseudo-wholemount" of euxassay_016795. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016795_01 euxassay_016795_02 euxassay_016795_03 euxassay_016795_04
EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607
euxassay_016795_05 euxassay_016795_06 euxassay_016795_07 euxassay_016795_08 euxassay_016795_09
EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607
euxassay_016795_10 euxassay_016795_11 euxassay_016795_12 euxassay_016795_13 euxassay_016795_14
EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607 EMAGE:16607
euxassay_016795_15 euxassay_016795_16 euxassay_016795_17 euxassay_016795_18 euxassay_016795_19
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euxassay_016795_20 euxassay_016795_21 euxassay_016795_22 euxassay_016795_23 euxassay_016795_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16607Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16607_wholemount_strong.wlz
16607_wholemount_moderate.wlz
16607_wholemount_weak.wlz
16607_wholemount_possible.wlz
16607_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16607_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63302
Entity Detected:Gnaq, guanine nucleotide binding protein, alpha q polypeptide ( MGI:95776)
Sequence:sense strand is shown

>T63302
CATTCCTTTCCTTCTTCCTCCTCCCCTTTCCTTTTGACCAAAACGAAGCTGATTTTTCTTCTTCCTGCTC
CTTCCCTCCCTGCTGATTTTATTCTCCCTCACTACCATGGCCTTGCCCCCAATCCCATTCTTGGCCAGTT
TCCCCCAATCCCATTCTTGGGCAGTTTCTCCCATCGAGTGACGTAGTCAGGACGGTGTTATTCGACTGAA
GCCGCCTTACGTCAGCTTCCTTTACCCGTGGGTCTGCTGAAGGCAGGAGCATAGGTGTTCGTACTATGGT
TTTAAATGGTTCTCTGGATGCACATCACATCTAGCATTTCTTTTTCAATACATACATACATACATACATA
CATATATATGTATACTATATATATTGTCTTGTCTCACTTGCACTAGGACAGTGGGTACCCATTGATGATC
AAGAGTCGTATCTTTTGGGTGCAGACCTTCAGCCACAGCAGGATTGTTAAGAGAAATGGATGGAGGTCAG
GGTGTGAGGGCACAGGCTGGGAAGAAGTCCTTGCTTCTCAAGGCCACGTACCGGCCGCGTCCTTCCACCC
TTGCCCTTTAAACCACAGATGCCAAATGATACGCCAACAGACACTACATTCCCCAGCAGCTGCTGCCAGA
GCCCTCTTGTAGCTTCTTTATTTTCTGTTTCTTTCCAGCTTTCCTACCCTCCTATCCCCCCTTGTGTTTG
GGCCACAATTTTGAAATAATTTTTATTATAGGTATGTGCTGCCAAAGCCAGATTTTTATAAGGTAAAATA
AATTAAGAATTTAAACAGTAAAAGCCAGTGTCTCAAAATGTCAGCATTAAAATGTGAAGGGGACAGCAGG
GTGTGAACCGGAAACACACATTGCCAAACAGTTGCCAACTGAACTGCTGCTTCTCATGGTCCGTTCTTTT
CTTTGCCCTTAAGGTCAATGCCAGTGTCCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236092. Forward Primer - name:165247_F_cDNA_Gnaq, sequence:CATTCCTTTCCTTCTTCCTCCT; Reverse Primer - name:165247_N_SP6_cDNA_Gnaq, sequence:CTGGACACTGGCATTGACCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16603 same embryo
 EMAGE:16605 same embryo
 EMAGE:16606 same embryo
 EMAGE:16604 same embryo
 EurExpress:euxassay_016795 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS