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EMAGE:1667

Vsx2 visual system homeobox 2 ( MGI:88401)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1667
0188b.jpg This embryo was treated with ProteinaseK for 30min.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1667Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1667_wholemount_strong_3D_1.wlz
1667_wholemount_possible_3D_1.wlz
1667_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1667_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalExpression was detected in ventral rhombencephalon.
future spinal cord
detected detected
regionalExpression was detected in ventral spinal cord.
eye
detected detected
The tissue was optic vesicle.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506058
Entity Detected:Vsx2, visual system homeobox 2 ( MGI:88401)
Sequence:sense strand is shown

>MGI:3506058
CAGCTGAGAAGCTCAGCCCACCGCAGCTGGAGGACATGGCTTAGGGCAAGGGCTGCTGGCCTCGGAACTG
CTCTTCTCCAGCCCTGCAGCCCTGCGGTGTGGCGAGTTCTCTCTGAGGCAAGGCCCATGTCTGGCTTCTG
CCACACCTCCCCACACCTCAGGCCCGCAATTACCCCCACGACTCTAGAAACAGACCAATTCTGTCCGTAG
CTTTGGACCATGCTGAGACATCCTGGACATACTCCCGATCCAGCGTCCTCAGCCTCAGCCCAGCTTCCCT
GCCTCCAAAGCCTTCTTGCTACCCCGACATCGAGGTCCTGCTACGTCATCCCTGAGAGACTCTTGTCCAA
GCCTATCCCTTATTCTCTGCTCTCTAAGCCTTCCTCCCCAGCTTAGCCCCCTCATGGGCGGCTGCGCCAC
TGCTGTAAACCCAGCCCACAGCCGGGCAGAGCTGTGATTGGATTGAGGCCCTGCCTCGGTGTCCCGAGCA
CCCCAGCACTTTGCAGGCAGGCTTTTCCCTCTTCACCTGTTCCTTCTCGCAGACCTGGACCCTGGCTGGC
TCCTGCTGTGTGAATGCACAGCCGGGTCACGTGGGTCCTGCTGTGAGGATGCACTGCTCAGCTGGTCCAC
GGTGTAACCTCTGCATTCTCTGCCCTGCCTGCTGATGGGCAGATTCAAGTCTAAACAGGGCAACTCTCAG
ATTGAGTCCTTCCCCAAACCTGTCTCACGCCGTGCTGT
nt 1043 - nt 1780 of NM_007701.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: CGCGGATCCAGCTGAGAAGCTCAGCCCACC 3'primer: CCCAAGCTTTACAGCACGGCGTGAGACAGG. Editors Note: Original information from the authors states that these two primers were used to generate a 737nt fragment (nt261-985 in NM_007701). Pairwise comparisons of these two primer sequences against the public versions of XM_126744 does identify a 737bp fragment however it is situated between nt1042-1781 of NM_007701.1
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508771 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE