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EMAGE:16698

Chpf chondroitin polymerizing factor ( MGI:106576)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698
"Pseudo-wholemount" of euxassay_016906. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016906_01 euxassay_016906_02 euxassay_016906_03 euxassay_016906_04
EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698
euxassay_016906_05 euxassay_016906_06 euxassay_016906_07 euxassay_016906_08 euxassay_016906_09
EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698
euxassay_016906_10 euxassay_016906_11 euxassay_016906_12 euxassay_016906_13 euxassay_016906_14
EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698 EMAGE:16698
euxassay_016906_15 euxassay_016906_16 euxassay_016906_17 euxassay_016906_18 euxassay_016906_19
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euxassay_016906_20 euxassay_016906_21 euxassay_016906_22 euxassay_016906_23 euxassay_016906_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16698Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16698_wholemount_strong.wlz
16698_wholemount_moderate.wlz
16698_wholemount_weak.wlz
16698_wholemount_possible.wlz
16698_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16698_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63542
Entity Detected:Chpf, chondroitin polymerizing factor ( MGI:106576)
Sequence:sense strand is shown

>T63542
CAGTGAGGCATGCTTCTACAACTCCGACTATGTGGCGGCCCGTGGCCGGCTGGTGGCGGCCTCGGAGCAG
GAGGAGGAGCTGCTGGAGAGCCTGGATGTGTACGAGTTGTTTCTGCGCTTCTCCAACTTGCACGTGCTGA
GAGCAGTAGAGCCAGCCTTGCTGCAGCGCTACCGGGCCCAGCCGTGCAGTGCACGGCTCAGTGAAGACCT
TTACCACCGCTGTCGCCAGAGCGTACTTGAGGGCCTTGGCTCCCGCACCCAGCTTGCCATGCTGCTCTTT
GAGCAGGAACAGGGGAACAGCACCTAAGCCCCTGCACCTGTCCCTGCTCTTCCCCAGGAACCTGGAGCCA
CGTGCCAGCCTCGCTGGACAGGGCTGGCTGTAGCCTCAGTCCCTAGGGCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 198113. Forward Primer - name:198113_F_exon_D1Bwg1363e, sequence:CAGTGAGGCATGCTTCTACAAC; Reverse Primer - name:198113_N_SP6_exon_D1Bwg1363e, sequence:CTGCCCTAGGGACTGAGGCTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16700 same embryo
 EMAGE:16701 same embryo
 EMAGE:16699 same embryo
 EMAGE:16702 same embryo
 EurExpress:euxassay_016906 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS