Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:1670

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1670 EMAGE:1670
0194b.jpg This embryo was treated with ProteinaseK for 30min. 0194a.jpg This embryo was treated with ProteinaseK for 3min.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1670Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1670_wholemount_moderate.wlz
1670_wholemount_possible.wlz
1670_wholemount_notDetected.wlz
1670_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1670_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
Expression was detected in dorsal and ventral diencephalon.
midbrain
detected detected
Expression was detected in a gradient in the mesencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506062
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Sequence:sense strand is shown

>MGI:3506062
CTTCTGTCACCATCCCCAGGCGTTAGCCATGGCTTCGGTCCTAGCTCCTGGCCAGCCCCGCTCCTTGGAC
TCCTCCAAACATAGACTGGAGGTGCATACAATCTCCGATACTTCCAGCCCTGAAGTCGCAGAGAAAGATA
AGGGCCAGCAAGGAAAGAATGAGGATGTGGGCGCCGAGGACCCGTCCAAGAAGAAGCGGCAACGCCGGCA
GAGGACTCATTTCACTAGCCAGCAGCTGCAGGAGCTGGAAGCCACTTTCCAGAGAAACCGCTACCCAGAC
ATGTCCACTCGCGAAGAAATCGCCGTGTGGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGA
ATCGCCGGGCCAAATGGAGAAAGCGGGAACGCAACCAGCAGGCCGAGCTGTGCAAGAATGGCTTTGGGCC
GCAGTTCAACGGGCTCATGCAGCCCTACGATGACATGTACCCCGGCTATTCGTACAACAATTGGGCTGCC
AAGGGCCTCACGTCAGCGTCTCTGTCCACCAAGAGCTTCCCCTTCTTCAACTCCATGAACGTCAATCCCC
TGTCCTCTCAGAGTATGTTTTCCCCGCCCAACTCCATCTCATCTATGAGTATGTCGTCCAGCATGGTGCC
CTCCGCGGTGACCGGCGTCCCGGGCTCCAGCCTCAATAGCCTGAATAACTTGAACAACCTGAGCAGCCCG
TCGCTGAATTCCGCGGTGCCCACGCCCGCCTGTCCTTACGCGCCGCCGACTCCTCCGTACGTTTATAGGG
ACACATGTAACTCGAGCCTGGCCAGCCTGAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAG
CGTGCAGAACCCGGCCTCCAACCTGAGTGCTTGCCAGTA
nt 681 - nt 1559 of NM_011098.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: cccaagcttcttctgtcaccatccccaggcg 3'primer: tgctctagatactggcaagcactcaggttgg
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508774 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE