EMAGE mouse gene expression spatial database

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EMAGE:16722

1700028P14Rik RIKEN cDNA 1700028P14 gene ( MGI:1914733)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016936. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016936_01	euxassay_016936_02	euxassay_016936_03	euxassay_016936_04

euxassay_016936_05	euxassay_016936_06	euxassay_016936_07	euxassay_016936_08	euxassay_016936_09

euxassay_016936_10	euxassay_016936_11	euxassay_016936_12	euxassay_016936_13	euxassay_016936_14

euxassay_016936_15	euxassay_016936_16	euxassay_016936_17	euxassay_016936_18	euxassay_016936_19

euxassay_016936_20	euxassay_016936_21	euxassay_016936_22	euxassay_016936_23	euxassay_016936_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16722_wholemount_strong.wlz

16722_wholemount_moderate.wlz

16722_wholemount_weak.wlz

16722_wholemount_possible.wlz

16722_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16722_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9805
Entity Detected:	1700028P14Rik, RIKEN cDNA 1700028P14 gene ( MGI:1914733)
Sequence:	sense strand is shown >T9805 TCCGTATAATGTAGAGCTGGACGGGCGCTGAGGGAAGTCCAGTCTGGTCTGTTCTCAACTGTAGCGCAGG CATGGGGACCTTTGATAGCCAATGTCACGAGTGCTTCGAGAGCAAAGAGCGGTGGTATGAGATTGGACCC ACGGATTTGCTGGAGCGCAAAGGCTCTCTAACTCTCCGCTCACATCACAAGAAATACTCGGAGCCAGTGT TGGTGTATTCCTGGCACCGTAACAGAGAAGCCTTCCCCAAAGATTATGATATAGAGAGTCTGGAGACGGT GAAAAACCTATGTATGTCAACATACAAGCGATTTGGAAGTGACAGTCCAAGGTGGATGTCGGAAGCACGT GAACAGATGGCCCAAGTTCTTGTAAACAAAGACTTGGTGGAGAAGAAGAAAACGGGCTTGCTGGATGAGG AAACACTATGCCCCGGGATTTTGGAAAGGGACCCTGAATTGCCTCCCACAGGCTTTCGGGATGTTCCTAT GGAATCCCCACCAGATCCGAGAAAACCGTGTTTTTCGACAACGTATTCTGAAGATTTCACTCCACAGTAT GAATATCCACCACCTGCCTGTCCTCGCCGAGAAGAGTACTCCATAGTCCACAGGAAGTGCCGTTCTCAAT TCACAGACCTCAATGGCTCCAAAAGACTTGGCATCAACACATGGCACGATGAGAGTGGAATTTATGCTAA TTCAGAGGCAAAACAAAAACTCTACGCATTGGCTAGAAATCCTATTGTCCCATTTTAAAAAAGCAATGTA TACAACCAGACCCTCATGCTAATGAAAAACGACAAATGTAGTAGTTAATAATTTTCATAATAAAAACAAA GCCTTCCAAATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6771302 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6771302 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16723 same embryo
	EMAGE:16726 same embryo
	EMAGE:16724 same embryo
	EMAGE:16725 same embryo
	EurExpress:euxassay_016936 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source