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EMAGE:16736

Mir493 microRNA 493 ( MGI:3718542)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736
"Pseudo-wholemount" of euxassay_019300. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019300_01 euxassay_019300_02 euxassay_019300_03 euxassay_019300_04
EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736
euxassay_019300_05 euxassay_019300_06 euxassay_019300_07 euxassay_019300_08 euxassay_019300_09
EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736
euxassay_019300_10 euxassay_019300_11 euxassay_019300_12 euxassay_019300_13 euxassay_019300_14
EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736
euxassay_019300_15 euxassay_019300_16 euxassay_019300_17 euxassay_019300_18 euxassay_019300_19
EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736 EMAGE:16736
euxassay_019300_20 euxassay_019300_21 euxassay_019300_22 euxassay_019300_23 euxassay_019300_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16736Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16736_wholemount_strong.wlz
16736_wholemount_moderate.wlz
16736_wholemount_weak.wlz
16736_wholemount_possible.wlz
16736_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16736_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
humerus
moderate moderate
regionalmoderate expression: see section 01 22 23 24 weak expression: see section 02 20 21
fibula
weak weak
regionalweak expression: see section 02 03 04
tibia
weak weak
regionalweak expression: see section 03 04
femur
weak weak
regionalweak expression: see section 04 05 06 07 08 22 23 24
nasal septum
moderate moderate
regionalmoderate expression: see section 13
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 14 15 16 20 weak expression: see section 04 05 06 17 18 19
axial skeleton
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 05 06 07 08 09 14 15
basioccipital bone
moderate moderate
regionalmoderate expression: see section 09 13 weak expression: see section 08 14
exoccipital bone
moderate moderate
regionalmoderate expression: see section 17 18 19
temporal bone
moderate moderate
regionalmoderate expression: see section 19 20 21 22
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 19 20 22 weak expression: see section 10
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
scapula
weak weak
regionalweak expression: see section 01 02 19 20
pelvic girdle skeleton
weak weak
regionalweak expression: see section 11 18 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70065
Entity Detected:Mir493, microRNA 493 ( MGI:3718542)
Sequence:sense strand is shown

>T70065
TGAAGGTCCTACTGTGTGCCAGG
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-493 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16739 same embryo
 EMAGE:16738 same embryo
 EMAGE:16735 same embryo
 EMAGE:16737 same embryo
 EurExpress:euxassay_019300 same experiment
 MGI:4826318 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS