EMAGE mouse gene expression spatial database

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EMAGE:16741

Mir501 microRNA 501 ( MGI:3629949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019290. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019290_01	euxassay_019290_02	euxassay_019290_03	euxassay_019290_04

euxassay_019290_05	euxassay_019290_06	euxassay_019290_07	euxassay_019290_08	euxassay_019290_09

euxassay_019290_10	euxassay_019290_11	euxassay_019290_12	euxassay_019290_13	euxassay_019290_14

euxassay_019290_15	euxassay_019290_16	euxassay_019290_17	euxassay_019290_18	euxassay_019290_19

euxassay_019290_20	euxassay_019290_21	euxassay_019290_22	euxassay_019290_23	euxassay_019290_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16741_wholemount_strong.wlz

16741_wholemount_moderate.wlz

16741_wholemount_weak.wlz

16741_wholemount_possible.wlz

16741_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16741_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 17 18 21 22

humerus

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 22 23 24

fibula

weak

regional

weak expression: see section 03 04 05

tibia

weak

regional

weak expression: see section 05

femur

weak

regional

weak expression: see section 05 06 07 09 10 24

meckel's cartilage

weak

regional

weak expression: see section 07 08 09 10 11 14 15 16 18 19 20

axial skeleton

weak

regional

weak expression: see section 09 10 13 14 15 16 17 18

basioccipital bone

weak

regional

weak expression: see section 09 10 11 12 13 14 15

exoccipital bone

weak

regional

weak expression: see section 02 03 04 05 20 21 22

temporal bone petrous part

weak

regional

weak expression: see section 01 02 03 20 21 22 23 24

orbito-sphenoid

weak

regional

weak expression: see section 02 03 04 05 07 08 09 11 13 14 15 20 21 24

viscerocranium

weak

regional

Expression in the turbinate bone.

pelvic girdle skeleton

moderate

regional

moderate expression: see section 13 weak expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70073
Entity Detected:	Mir501, microRNA 501 ( MGI:3629949)
Sequence:	sense strand is shown >T70073 AATCCTTTGTCCCTGGGTGAAA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-501-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16742 same embryo
	EMAGE:16740 same embryo
	EurExpress:euxassay_019290 same experiment
	MGI:4826322 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source