EMAGE mouse gene expression spatial database

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EMAGE:16743

Mir511 microRNA 511 ( MGI:3718546)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019295. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019295_01	euxassay_019295_02	euxassay_019295_03	euxassay_019295_04

euxassay_019295_05	euxassay_019295_06	euxassay_019295_07	euxassay_019295_08	euxassay_019295_09

euxassay_019295_10	euxassay_019295_11	euxassay_019295_12	euxassay_019295_13	euxassay_019295_14

euxassay_019295_15	euxassay_019295_16	euxassay_019295_17	euxassay_019295_18	euxassay_019295_19

euxassay_019295_20	euxassay_019295_21	euxassay_019295_22	euxassay_019295_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16743_wholemount_strong.wlz

16743_wholemount_moderate.wlz

16743_wholemount_weak.wlz

16743_wholemount_possible.wlz

16743_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16743_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

weak

regional

weak expression: see section 01 02 20 21 22

fibula

weak

regional

weak expression: see section 23

tibia

weak

regional

weak expression: see section 23

femur

weak

regional

weak expression: see section 03 16 17 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70079
Entity Detected:	Mir511, microRNA 511 ( MGI:3718546)
Sequence:	sense strand is shown >T70079 ATGCCTTTTGCTCTGCACTCA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-511 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16747 same embryo
	EMAGE:16744 same embryo
	EMAGE:16745 same embryo
	EMAGE:16746 same embryo
	EurExpress:euxassay_019295 same experiment
	MGI:4826328 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source