EMAGE mouse gene expression spatial database

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EMAGE:16747

Mir509 microRNA 509 ( MGI:3718545)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019293. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019293_01	euxassay_019293_02	euxassay_019293_03	euxassay_019293_04

euxassay_019293_05	euxassay_019293_06	euxassay_019293_07	euxassay_019293_08	euxassay_019293_09

euxassay_019293_10	euxassay_019293_11	euxassay_019293_12	euxassay_019293_13	euxassay_019293_14

euxassay_019293_15	euxassay_019293_16	euxassay_019293_17	euxassay_019293_18	euxassay_019293_19

euxassay_019293_20	euxassay_019293_21	euxassay_019293_22	euxassay_019293_23	euxassay_019293_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16747_wholemount_strong.wlz

16747_wholemount_moderate.wlz

16747_wholemount_weak.wlz

16747_wholemount_possible.wlz

16747_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16747_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 01 02 22 23 24 weak expression: see section 03 21

fibula

weak

regional

weak expression: see section 24

tibia

weak

regional

weak expression: see section 24

femur

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 17 18 19 21 22

axial skeleton

moderate

regional

moderate expression: see section 10 weak expression: see section 09 11 12

basioccipital bone

weak

regional

weak expression: see section 14 15 16

temporal bone

weak

regional

weak expression: see section 22 23

orbito-sphenoid

weak

regional

weak expression: see section 15 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70077
Entity Detected:	Mir509, microRNA 509 ( MGI:3718545)
Sequence:	sense strand is shown >T70077 TGATTGACATTTCTGTAATGG
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-509-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16744 same embryo
	EMAGE:16743 same embryo
	EMAGE:16745 same embryo
	EMAGE:16746 same embryo
	EurExpress:euxassay_019293 same experiment
	MGI:4826326 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source