EMAGE mouse gene expression spatial database

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EMAGE:16790

Stmn1 stathmin 1 ( MGI:96739)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008627. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008627_01	euxassay_008627_02	euxassay_008627_03	euxassay_008627_04

euxassay_008627_05	euxassay_008627_06	euxassay_008627_07	euxassay_008627_08	euxassay_008627_09

euxassay_008627_10	euxassay_008627_11	euxassay_008627_12	euxassay_008627_13	euxassay_008627_14

euxassay_008627_15	euxassay_008627_16	euxassay_008627_17	euxassay_008627_18	euxassay_008627_19

euxassay_008627_20	euxassay_008627_21	euxassay_008627_22	euxassay_008627_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16790_wholemount_strong.wlz

16790_wholemount_moderate.wlz

16790_wholemount_weak.wlz

16790_wholemount_possible.wlz

16790_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16790_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 17 18 19

trigeminal v nerve

strong

regional

strong expression: see section 09 16

spinal cord

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 08 14 15

cervical ganglion

strong

regional

strong expression: see section 08 16

thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

retina

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1315
Entity Detected:	Stmn1, stathmin 1 ( MGI:96739)
Sequence:	sense strand is shown >T1315 AGCACTGTTGGCCTACTGGNAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGCCAGTGGA TTGTGTAGAGTATACAGCCAGTCTCTTGTCTTCTGTTCGACATGGCATCTTCTGATATTCAGGTGAAAGA GCTGGAGAAGCGCGCTTCAGGCCAGGCTTTTGAGCTGATTCTCAGCCCTCGGTCAAAAGAATCTGTCCCC GATTTCCCCCTTTCCCCCCCAAAGAAGAAGGACCTTTCCCTGGAGGAAATCCAGAAGAAATTAGAAGCTG CAGAAGAAAGACGCAAGTCTCATGAGGCGGAAGTCTTGAAGCAGCTCGCGGAGAAGCGGGAGCATGAGAA GGAGGTGCTCCAGAAAGCCATCGAGGAGAACAACAACTTCAGCAAGATGGCGGAGGAGAAGCTGACCCAC AAAATGGAGGCTAACAAAGAGAACCGGGAGGCGCAGATGGCGGCCAAGCTGGAGCGCTTGCGAGAGAAGG ACAAGCACGTGGAAGAGGTGCGGAAGAACAAAGAATCCAAAGACCCCGCGGATGAGACTGAGGCTG
Notes:	The probe template was PCR amplified from IMAGE:2259315 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2259315 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16789 same embryo
	EMAGE:16788 same embryo
	EMAGE:16787 same embryo
	EurExpress:euxassay_008627 same experiment
	MGI:4828507 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source