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EMAGE:16794

Prc1 protein regulator of cytokinesis 1 ( MGI:1858961)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794
"Pseudo-wholemount" of euxassay_008791. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008791_01 euxassay_008791_02 euxassay_008791_03 euxassay_008791_04
EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794
euxassay_008791_05 euxassay_008791_06 euxassay_008791_07 euxassay_008791_08 euxassay_008791_09
EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794
euxassay_008791_10 euxassay_008791_11 euxassay_008791_12 euxassay_008791_13 euxassay_008791_14
EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794 EMAGE:16794
euxassay_008791_15 euxassay_008791_16 euxassay_008791_17 euxassay_008791_18 euxassay_008791_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16794Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16794_wholemount_strong.wlz
16794_wholemount_moderate.wlz
16794_wholemount_weak.wlz
16794_wholemount_possible.wlz
16794_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16794_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1282
Entity Detected:Prc1, protein regulator of cytokinesis 1 ( MGI:1858961)
Sequence:sense strand is shown

>T1282
TCCTCGAGCCTGTTGGCCTACTGGCAGAAGTTGCTGAAGCAGCTGGAAATGAAAAAATCACAAAATGAAG
CAGAATGTGAGGGGCTCCGCACTCAAATCCGAGAGCTCTGGGATAGGTTACAAATACCTGAAGAAGAGAG
AGAGCCTGTGGAGGCAATTATGACTGGATCAAAAACCAAAATCAGGAATGCGCTGAAATTAGAAGTGGAT
CGGTTAGAAGAACTGAAAATGCAAAACATAAAGCAAGTGATTGAGAAAATCCGAGTGGAGCTGGCTCAAT
TCTGGGACCAGTGTTTTTATAGCCAGGAACAGAGGCAGGCTTTTGCCCCTTACTATTCTGAGGACTACAC
AGAAAACCTGCTCCATCTTCATGATGCCGAGATTGTACGGTTAAGAAACTACTATGACGTTCACAAGGAG
CTGTTCCAAGGCGTCCAGAAGTGGGAAGAGAGCCGGAAACTTTTCCTAGAGTTTGAGAGAAAAGCTTCAG
GTCCAGGTCGATTTACAAACCGAGGGGGAAATCTTTTAAAAGAAGAAAAGGAACGAGCAAAGCTCCAGAA
AACACTCCCTAAGCTGGAAGAGGAGT
Notes:The probe template was PCR amplified from IMAGE:2225848 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225848 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BL6/SV129
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16793 same embryo
 EMAGE:16792 same embryo
 EurExpress:euxassay_008791 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS