EMAGE mouse gene expression spatial database

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EMAGE:16806

Tnnc2 troponin C2, fast ( MGI:98780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008629. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008629_01	euxassay_008629_02	euxassay_008629_03	euxassay_008629_04

euxassay_008629_05	euxassay_008629_06	euxassay_008629_07	euxassay_008629_08	euxassay_008629_09

euxassay_008629_10	euxassay_008629_11	euxassay_008629_12	euxassay_008629_13	euxassay_008629_14

euxassay_008629_15	euxassay_008629_16	euxassay_008629_17	euxassay_008629_18	euxassay_008629_19

euxassay_008629_20	euxassay_008629_21	euxassay_008629_22	euxassay_008629_23	euxassay_008629_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16806_wholemount_strong.wlz

16806_wholemount_moderate.wlz

16806_wholemount_weak.wlz

16806_wholemount_possible.wlz

16806_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16806_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22 23 24

upper leg muscle

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 18 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 23 24

hand

strong

regional

strong expression: see section 03 04 05 06 07 23 24

foot

strong

regional

strong expression: see section 06 07 08 09 10 17 18 19 20 21

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

eye skeletal muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 16 17 18 19 20 21 22 23 24

tongue muscle

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1308
Entity Detected:	Tnnc2, troponin C2, fast ( MGI:98780)
Sequence:	sense strand is shown >T1308 TCTCGAGCCTAGCTCGAGTCGGCCTTGTTGGCCTACTGGAGATCTGGGGCACCCTTGGGTGGTGGAGTGC GGAGGAGACAACCCACAGCGGAGGAGTCCCAGTCGCCAGCAACCATGACGGACCAACAGGCTGAGGCCAG GTCCTACCTCAGCGAGGAGATGATCGCTGAGTTCAAGGCTGCCTTTGACATGTTCGATGCTGATGGCGGT GGGGACATCAGCGTTAAAGAGTTGGGCACCGTGATGAGGATGCTAGGGCAGACACCCACCAAAGAGGAAT TGGATGCCATCATCGAGGAGGTGGACGAGGATGGCAGCGGTACTATCGACTTTGAAGAGTTCTTGGTCAT GATGGTGCGCCAGATGAAAGAGGATGCGAAGGGGAAGAGCGAAGAGGAACTGGCTGAGTGCTTCCGCATC TTTGACAGGAACGCAGACGGCTACATTGATGCTGAGGAGCTAGCTGAGATTTTCCGGGCTTCTGGGGAGC ATGTGACAGAAGAGGAGATCGAATCCCTGATGAAGGATGGTGATAAAAACAACGACGGCCGCATTGACTT TGATGAGTTTCTGAAGATGA
Notes:	The probe template was PCR amplified from IMAGE:2259086 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2259086 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16804 same embryo
	EMAGE:16807 same embryo
	EMAGE:16803 same embryo
	EMAGE:16805 same embryo
	EurExpress:euxassay_008629 same experiment
	MGI:4828857 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source