EMAGE mouse gene expression spatial database

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EMAGE:16810

Meox2 mesenchyme homeobox 2 ( MGI:103219)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008785. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008785_01	euxassay_008785_02	euxassay_008785_03	euxassay_008785_04

euxassay_008785_05	euxassay_008785_06	euxassay_008785_07	euxassay_008785_08	euxassay_008785_09

euxassay_008785_10	euxassay_008785_11	euxassay_008785_12	euxassay_008785_13	euxassay_008785_14

euxassay_008785_15	euxassay_008785_16	euxassay_008785_17	euxassay_008785_18	euxassay_008785_19

euxassay_008785_20	euxassay_008785_21	euxassay_008785_22	euxassay_008785_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16810_wholemount_strong.wlz

16810_wholemount_moderate.wlz

16810_wholemount_weak.wlz

16810_wholemount_possible.wlz

16810_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16810_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 weak expression: see section 17 18

forearm mesenchyme

moderate

regional

moderate expression: see section 01 02

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 11 14 21 22 23

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 11 14 21 22 23

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 11 14 21 22 23

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 11 14 21 22 23

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 22 23

foot mesenchyme

moderate

regional

moderate expression: see section 05 06

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 23

vibrissa

strong

regional

strong expression: see section 04 05 moderate expression: see section 06 07 21 22 23

cochlea

moderate

regional

moderate expression: see section 18 19 22 23 weak expression: see section 20

esophagus

moderate

regional

moderate expression: see section 12 13 14

tongue

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

stomach

weak

regional

weak expression: see section 02 03 04 05 06

palatal shelf

moderate

regional

moderate expression: see section 11 12 13 14 15

metanephros

moderate

regional

moderate expression: see section 06 07 08 09 10 18 19 weak expression: see section 15 16 17

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 22 23

axial skeleton

weak

regional

weak expression: see section 10 11 14

exoccipital bone

moderate

regional

moderate expression: see section 01 02 03 04 05 17 18 19 20 21

vault of skull

moderate

regional

moderate expression: see section 22 23

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 20 21 22 23

clavicle

weak

regional

weak expression: see section 08 09 10 11 16

tail mesenchyme

strong

regional

strong expression: see section 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2812
Entity Detected:	Meox2, mesenchyme homeobox 2 ( MGI:103219)
Sequence:	sense strand is shown >T2812 CTCNAGGNCANAATTCGNNACGAGGTGAGATAGCAGTGAATCTAGACCTCACTGAAAGACAGGTGAAAGT GTGGTTCCAGAACAGGAGAATGAAGTGGAAGCGGGTCAAAGGGGGACAGCAAGGAGCTGCTGCCCGAGAA AAGGAACTGGTGAATGTGAAAAAGGGAACGCTTCTTCCGTCAGAGCTGTCAGGAATCGGTGCAGCCACCC TCCAGCAGACAGGGGACTCACTAGCAAACGAGGACAGCCGCGATAGTGACCACAGCTCTGAGCACGCACA CTTATGATACCTACAAAGACCAGCTCCATTCTCAGGAAAGCACCATTGTGATGGCAAACCTTACCGAACA TCGTTTACATGGCAGATGTCTGTGGCAGTGTGGCTTAGTATAATTAAATGCAGGCATCTCGAGTCTGTGT CTCATGATGGATAGACGGTTTACACTAAGTGCCTTTTATTGAAGATGCTTCCACAGTGAAATTGGAGAAA GCGAACGTATTCCAAATACACTTGTTCCTATATATGACAGAGAGGGAGATGGATGTTTGCTTTGGCTTGC ACTGAAAATTAA
Notes:	The probe template was PCR amplified from IMAGE:1528058 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1528058 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826161 same experiment
	EurExpress:euxassay_008785 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source