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EMAGE:16815

Bmp3 bone morphogenetic protein 3 ( MGI:88179)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815
"Pseudo-wholemount" of euxassay_008763. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008763_01 euxassay_008763_02 euxassay_008763_03 euxassay_008763_04
EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815
euxassay_008763_05 euxassay_008763_06 euxassay_008763_07 euxassay_008763_08 euxassay_008763_09
EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815
euxassay_008763_10 euxassay_008763_11 euxassay_008763_12 euxassay_008763_13 euxassay_008763_14
EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815 EMAGE:16815
euxassay_008763_15 euxassay_008763_16 euxassay_008763_17 euxassay_008763_18 euxassay_008763_19
EMAGE:16815 EMAGE:16815
euxassay_008763_20 euxassay_008763_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16815Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16815_wholemount_strong.wlz
16815_wholemount_moderate.wlz
16815_wholemount_weak.wlz
16815_wholemount_possible.wlz
16815_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16815_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 15 16 17 moderate expression: see section 01 02 03 04 18 19 20
hand mesenchyme
strong strong
regionalstrong expression: see section 18 19 20 21
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 16
hindlimb digit 1 metatarsal
strong strong
regionalstrong expression: see section 17
hindlimb digit 2 metatarsal
strong strong
regionalstrong expression: see section 15 17
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 15 16
hindlimb digit 3 metatarsal
strong strong
regionalstrong expression: see section 15 16 17
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 15 16
hindlimb digit 4 metatarsal
strong strong
regionalstrong expression: see section 15 16 17
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 15 16
hindlimb digit 5 metatarsal
strong strong
regionalstrong expression: see section 15 16 17
hindlimb digit 5 phalanx
strong strong
regionalstrong expression: see section 15 16
foot mesenchyme
strong strong
regionalstrong expression: see section 18
axial musculature
strong strong
regionalstrong expression: see section 01 03 14 18 19 20 21
vibrissa
strong strong
regionalstrong expression: see section 02 03 04 05 06 17 18 19 20 21
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 02 03 04 06 07 08 weak expression: see section 05
rest of cerebellum mantle layer
strong strong
regionalstrong expression: see section 09 16 17 18 19 20 moderate expression: see section 04 08 weak expression: see section 05 06 07 10 11 14 15
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 08 moderate expression: see section 07 13 14
cervical ganglion
strong strong
regionalstrong expression: see section 15 moderate expression: see section 07 08 16
thoracic ganglion
strong strong
regionalstrong expression: see section 08
pharyngo-tympanic tube
strong strong
regionalstrong expression: see section 01 02 03 04 19 20 21
extrinsic ocular muscle
strong strong
regionalstrong expression: see section 03 04 05 06 18 20
nasal septum
strong strong
regionalstrong expression: see section 10 11 12 13 14
viscerocranium
strong strong
regionalExpression in the turbinate bone.
tongue muscle
moderate moderate
regionalmoderate expression: see section 12
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07
midgut
strong strong
regionalstrong expression: see section 11 12 13 14 moderate expression: see section 05 06 07 08 09 10
lower lip
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
mandible
moderate moderate
regionalmoderate expression: see section 03 04 05 06 15 16 17 18 19 20 21
upper lip
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 moderate expression: see section 04
maxilla
moderate moderate
regionalmoderate expression: see section 03 04 05 06 15 16 17 18 19
palatal shelf
strong strong
regionalstrong expression: see section 10 13 moderate expression: see section 09
metanephros
moderate moderate
regionalmoderate expression: see section 03 04 06 12 13 14 weak expression: see section 05
ureter
moderate moderate
regionalmoderate expression: see section 06 09
urethra of male
moderate moderate
regionalmoderate expression: see section 07 08
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19
axial skeleton
strong strong
regionalstrong expression: see section 13 14 15 moderate expression: see section 07 10 11
nucleus pulposus
moderate moderate
regionalmoderate expression: see section 06 07 08 10 11 12
temporal bone
strong strong
regionalstrong expression: see section 03 04 19 20 21 moderate expression: see section 01 02
orbito-sphenoid
strong strong
regionalstrong expression: see section 03 04 12 13 19 20 21 moderate expression: see section 01 02
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1247
Entity Detected:Bmp3, bone morphogenetic protein 3 ( MGI:88179)
Sequence:sense strand is shown

>T1247
TCCTCGAGACTGTTGGCCTACTGGTTCTCTCTTCTGGAGGTAGTCTTGCTCCAGCTGCCTTAGGAAGGCG
AGCCAGAGGCTAGAGGGAGAACTCGCTGCAGGCGGACCGGACAGATCAGATCAGGACAAGAAGGAACAGA
ACCAGTCTCTCTATCTCTGTCTCTCTGTTCGTCACTGTCTCTGTGTCTCTGTCTCCCTGTCTCCCTCTTT
CTCTGTCTGTCTGTCTGTCTTCACACACACACACACACACACACACACACACACCACACGTCCTCTGAGA
ACTCAGCTGGCTGCGACAGCGCACACCCCGCCCCGGCACGCGTCCCAGGCTGGTGGAGCGCAGATCCAGA
TCCCCATAGCGGCAGCCCGGCCGGGTGTTCCCAGTCTCAGGTGGCACGGAAGGGAGAGCCCGCGCTTGAC
AACTCGGCTGCGCTCGCCCAGACCTTCCGCCCTCCGGCTGGCCGGCGAGAGGAGCTGAGCGGGCGCGGCT
GCCACCCAGAGCCCACCTGCCGACCCTCGCGGCCTCGCTCCAGCTGTGCCCGGCTCTCAGTGTCCCGCTC
CGCAGCTGGAGCACCACCCCG
Notes:The probe template was PCR amplified from IMAGE:2225264 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225264 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16816 same embryo
 EMAGE:16814 same embryo
 EurExpress:euxassay_008763 same experiment
 MGI:4823484 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS