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EMAGE:16825

Bap1 Brca1 associated protein 1 ( MGI:1206586)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16825
euxassay_008636_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16825Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16825_wholemount_strong.wlz
16825_wholemount_moderate.wlz
16825_wholemount_weak.wlz
16825_wholemount_possible.wlz
16825_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16825_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2821
Entity Detected:Bap1, Brca1 associated protein 1 ( MGI:1206586)
Sequence:sense strand is shown

>T2821
CTCNAGCCAGATTCGGCACGAGGCACAAGCAGCGGAAGCCTGACCGGCGGAAACGCTCTCGCCCCTACAA
GGCCAAACGCCAGTGAGAACCTCTGGCCCTGACTCTGCAGCCTCTCTTGCCGCAAGGCCCTCATCAGGGC
CCTTCACTGTCCCTCTTTTCCAAGTATTACTGAGTAGTTCAACGAGAGCCCAGGTCCTGGGAGTGGGAGT
GACATGTATTCTGCACTGTGCTCTGGGGTCTGGCAGGGACAAGGCAGCTCCTCAATGCTCAGGAAGCAGC
AGCTGGAACTGGAGGGCATCCACCATGGCCTCCACAGCCATCTGAGGAGCAGCAGGACCTGGCCTTTCTG
CCTGGGCAGCAGGATGTACATTCTACCTATTGGAGATGTTTAGGTTTTGGGCTTTTGTCCATCTTGCCAC
TGTATTAACATGGCAGCTTCCTGACTCTGCTGTCTCTCCCAGCAGCTGTCATTCTGCTGGGCCAGGTCTC
TTAGAATACATCACAGTCCCAGCTGAGAAACACACCACCACTAGCCTGTCCTCGTTGGGAAGAAGCTTGG
CCTACA
Notes:The probe template was PCR amplified from IMAGE:1528355 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1528355 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16826 same embryo
 EMAGE:16823 same embryo
 EMAGE:16824 same embryo
 EurExpress:euxassay_008636 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS