EMAGE mouse gene expression spatial database

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EMAGE:16861

Mep1a meprin 1 alpha ( MGI:96963)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008632. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008632_01	euxassay_008632_02	euxassay_008632_03	euxassay_008632_04

euxassay_008632_05	euxassay_008632_06	euxassay_008632_07	euxassay_008632_08	euxassay_008632_09

euxassay_008632_10	euxassay_008632_11	euxassay_008632_12	euxassay_008632_13	euxassay_008632_14

euxassay_008632_15

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16861_wholemount_strong.wlz

16861_wholemount_moderate.wlz

16861_wholemount_weak.wlz

16861_wholemount_possible.wlz

16861_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16861_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midgut

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

kidney calyx

strong

regional

strong expression: see section 01 02 03 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1301
Entity Detected:	Mep1a, meprin 1 alpha ( MGI:96963)
Sequence:	sense strand is shown >T1301 CCTCNAGCCTGTTGGCCCACTGGATTTTGCAATAATGCTTTGGATACAACCTGCGTGTCTTCTCTCACTG ATTTTTTCTGCCCACATAGCAGCTGTATCGATTAAGCATCTTTTGAATGGATCTGATCACGATACAGATG TTGGTGAACAGAAGGATATTTTCGAAATCAATTTAGCTGCAGGCCTGAACCTCTTTCAAGGGGACATCCT TCTGCCCAGAACCAGAAATGCCATGCGAGATCCCTCAAGCAGATGGAAGCTCCCCATTCCATACATTCTG GCTGACAATCTGGAGCTGAATGCCAAAGGAGCCATCCTCCATGCCTTTGAGATGTTCCGCCTCAAGTCTT GTGTGGATTTCAAGCCCTACGAAGGGGAGAGTTCGTATATCATCTTCCAGAAACTTTCTGGGTGCTGGTC TATGATTGGTGATCAACAGGTGGGACAGAACATTTCCATTGGTGAGGGATGTGACTTTAAGGCCACCATT GAACATGAAATCCTGCATGCTCTGGGATTCTTCCATGAGCAGTCAAGGACTGACCGGGATGATTATGTGA ACATTTGGTGG
Notes:	The probe template was PCR amplified from IMAGE:2247466 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2247466 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16857 same embryo
	EMAGE:16860 same embryo
	EMAGE:16859 same embryo
	EMAGE:16858 same embryo
	EMAGE:16856 same embryo
	EurExpress:euxassay_008632 same experiment
	MGI:4826162 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source