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EMAGE:16924

Plscr1 phospholipid scramblase 1 ( MGI:893575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16924 EMAGE:16924 EMAGE:16924 EMAGE:16924 EMAGE:16924
"Pseudo-wholemount" of euxassay_008744. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008744_01 euxassay_008744_02 euxassay_008744_03 euxassay_008744_04
EMAGE:16924 EMAGE:16924 EMAGE:16924 EMAGE:16924 EMAGE:16924
euxassay_008744_05 euxassay_008744_06 euxassay_008744_07 euxassay_008744_08 euxassay_008744_09
EMAGE:16924 EMAGE:16924 EMAGE:16924
euxassay_008744_10 euxassay_008744_11 euxassay_008744_12

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16924Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16924_wholemount_strong.wlz
16924_wholemount_moderate.wlz
16924_wholemount_weak.wlz
16924_wholemount_possible.wlz
16924_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16924_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindgut
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11
midgut
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11
kidney calyx
moderate moderate
regionalmoderate expression: see section 01 02 03 04 08 09 10 11 12
kidney pelvis
moderate moderate
regionalmoderate expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3161
Entity Detected:Plscr1, phospholipid scramblase 1 ( MGI:893575)
Sequence:sense strand is shown

>T3161
TGGCCTCGAGCCAGATTCGGCACGAGGTGACTCAGACCTGGCACCCATGTCTGCCCAAGTTCACTCTCCA
AAATGAGAAGAAGCAGGATGTCCTGAAAGTAGTTGGTCCGTGTGTTGTGTGTAGCTGCTGTTCCGACATT
GACTTTGAGCTCAAATCTCTAGATGAAGAATCAGTAGTTGGCAAAATTTCTAAGCAGTGGTCTGGTTTTG
TGAGAGAGGCCTTCACGGATGCAGACAATTTTGGGATCCAGTTCCCGCTAGACCTGGATGTGAAGATGAA
AGCTGTGATGCTTGGTGCTTGTTTCCTCATAGATTTCATGTTTTTTGAAAGAACTGGAAACGAGGAGCAA
AGATCAGGAGCATGGCAGTAACTCCCTGAGAGTTCTTGAGGTTTAAGGACGACAACTTTATGGACCCTGA
ATGGAAACTGAGGAATCACAAGGCACACACCGTGGCTTCTTTTCCTTTACTGAAATAACTTTCTATCAAC
TCACCTGTGATGCCTGGGTGCCCTGTTGTACAATTATGCTCCCAAATTAGAGTTTATTTT
Notes:The probe template was PCR amplified from IMAGE:1548204 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1548204 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16928 same embryo
 EMAGE:16925 same embryo
 EMAGE:16926 same embryo
 EMAGE:16929 same embryo
 EMAGE:16927 same embryo
 EurExpress:euxassay_008744 same experiment
 MGI:4827285 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS