EMAGE mouse gene expression spatial database

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EMAGE:16925

Hdc histidine decarboxylase ( MGI:96062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008736. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008736_01	euxassay_008736_02	euxassay_008736_03	euxassay_008736_04

euxassay_008736_05	euxassay_008736_06	euxassay_008736_07	euxassay_008736_08	euxassay_008736_09

euxassay_008736_10	euxassay_008736_11	euxassay_008736_12	euxassay_008736_13	euxassay_008736_14

euxassay_008736_15	euxassay_008736_16	euxassay_008736_17	euxassay_008736_18	euxassay_008736_19

euxassay_008736_20	euxassay_008736_21	euxassay_008736_22	euxassay_008736_23	euxassay_008736_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16925_wholemount_strong.wlz

16925_wholemount_moderate.wlz

16925_wholemount_weak.wlz

16925_wholemount_possible.wlz

16925_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16925_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 13 14 15 21 22 23 24 moderate expression: see section 10 11 12 16 17 18 19 20

lateral ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 09 10 12 13 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

heart atrium

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 10 11 12

heart ventricle

strong

regional

strong expression: see section 13 14 15 16 17 18 19 moderate expression: see section 11 12

tongue muscle

strong

regional

strong expression: see section 13 14 moderate expression: see section 10 11 12 15

metanephros

strong

regional

strong expression: see section 08 09 10 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1275
Entity Detected:	Hdc, histidine decarboxylase ( MGI:96062)
Sequence:	sense strand is shown >T1275 TCCTCGAGCACTGTTGGCCTACTGGAGTGCACAGCACAGACAAAGGCGACCAAGAAGCCCTGTTGACAGC TGCCTTCCAGCCTCCTCTGTCTGTCTGCCAGGAGGAGCAATCCAAGGGAGATGATGGAGCCCTGTGAATA CCGTGAATACCGTGAATACTACCGAGCTAGAGGGAAAGAGATGGTGGATTACATCTCCCAGTACCTGAGC ACTGTGCGGGAGAGGCAGGTGACTCCAAATGTGCAGCCTGGATACCTGCGAGCCCAGCTACCTGCGAGTG CTCCCGAGGAACCCGACAGCTGGGACAGCATCTTTGGGGACATTGAACGAGTCATCATGCCTGGGGTGGT TCACTGGCAGAGCCCCCACATGCACGCCTACTATCCTGCTCTTACCTCATGGCCATCCCTGCTAGGAGAC ATGCTGGCCGATGCCATTAACTGCTTAGGATTCACGTGGGCATCTAGCCCAGCTTGCACAGAGCTGGAGA TGAACATCATGGACTGGCTGGCGAAGATGCTGGGGCTCCCGGAGTACTTCCTGCACCACCATCCCAGCAG CCGAGGGGG
Notes:	The probe template was PCR amplified from IMAGE:2225742 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225742 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16928 same embryo
	EMAGE:16924 same embryo
	EMAGE:16926 same embryo
	EMAGE:16929 same embryo
	EMAGE:16927 same embryo
	EurExpress:euxassay_008736 same experiment
	MGI:4825344 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source