EMAGE mouse gene expression spatial database

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EMAGE:16970

Lsp1 lymphocyte specific 1 ( MGI:96832)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007233. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007233_01	euxassay_007233_02	euxassay_007233_03	euxassay_007233_04

euxassay_007233_05	euxassay_007233_06	euxassay_007233_07	euxassay_007233_08	euxassay_007233_09

euxassay_007233_10	euxassay_007233_11	euxassay_007233_12	euxassay_007233_13	euxassay_007233_14

euxassay_007233_15	euxassay_007233_16	euxassay_007233_17	euxassay_007233_18	euxassay_007233_19

euxassay_007233_20	euxassay_007233_21	euxassay_007233_22	euxassay_007233_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16970_wholemount_strong.wlz

16970_wholemount_moderate.wlz

16970_wholemount_weak.wlz

16970_wholemount_possible.wlz

16970_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16970_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 11 12 weak expression: see section 10

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 11 12

rectum

moderate

regional

moderate expression: see section 11 12

midgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

lower jaw incisor

moderate

regional

moderate expression: see section 09 14 weak expression: see section 10 15

palatal shelf

moderate

regional

moderate expression: see section 10 11 13 14

upper jaw incisor

moderate

regional

moderate expression: see section 08 weak expression: see section 10 15

bladder

moderate

regional

moderate expression: see section 11 12 13 14

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3926
Entity Detected:	Lsp1, lymphocyte specific 1 ( MGI:96832)
Sequence:	sense strand is shown >T3926 GAAAAAAGGGAAGGGGAAAGAGGAGAAAGGGAAGAAGGCAGAGGAGAACAGAGGTGCTGGGCCAGAGCAC CAGGAGCCTTTGAAGCTGCAGGCTGTGTCCCCAGGGCCAGGGGTCAGGCCAGGTCATCAGCGTCGTCGGG CAGGCCTGCAGCATGAATGGCCCCGCACTCCTGAGGAGAAATGCTAGCAAACGGGGCCTGGAGAAGCTGC TGAGGCTCACAACTCAGTGGAGAGAAGAAGACGAGGAAGAGGCTGCCCGTGAGCAGCGTCAACGGGAGCG AGAGAGGCAGCTACAGGACCAGGACAAAGATAAAGAAGATGATGGTGGCCATTCCCTGGAACAGCCAGGA CAGCAGACACTCATCAGCCTGAAGTCCTCTGAACTGGATGAAGATGAGGGTTTTGGTGACTGGTCCCAAA AGCCGGAGCCACGGCAGCAATTCTGGGGGAATGAGGGGACTGCAGAAGGGACTGAACCCTCTCAAAGCGA GAGACCAGAGGAGAAACAAACAGAGGAGAGTTCTCACCAAGCCAAAGTCCACTTGGAGGAGTCAAACCTG AGCTACAGGGAGCCCGATCCAGAGGATGCTGTTGGGGGTTCTGGGGAGGCGGAAGAGGTCAGGACCCCCA GCCCTTTGGCCTTAGAAGACACCGTTGAGCTGAGTTCACCTCCCCTGAGCCCTACCACCAAACTGGCTGA TAGGACCGAGTCCCTGAACCGCTCCATTAAAAAGAGCAACAGTGTGAAGAAGTCTCAACCAACCTTGCCC ATTTCCACAATTGATGAACGCCTGCAGCAGTATACCCAGGCCACTGAGTCTTCTGGCCGAACTCCTAAGC TGTCCCGCCAGCCCTCCATAGAGCTGCCCAGCATGGCCGTAGCCAGTACCAAGACTCTTTGGGAAACAGG AGAAGTGCAGAGTCAGTCTGCTTCTAAGACACCCTCCTGCCAGGATATAGTAGCTGGAGACATGAGCAAG AAAAGTCTGTGGGAGCAGAAAGGAGGCTCCAAGATCTCATCCACCATCAAGAGCACCCCATCTGGAAAGC GGTACAAGTTCGTGGCCACTGGACATGGGAAGTACGAGAAAGTACTTGTGGATGAGGGCTCAGCACCATA GACCATGTTTGCATCCTGGATAGACTCCGGCCGGATGCCACTCCCAGCCCTGACCAAGAAATTGCTTCCT GTTGCCAGTCTGGCCTGCTCTCCTGCCTCCAACAACCAAGCTGCCGATAGCCTCTCCTTTTGCTCCTGGA CCCTCCATCTTCTCTTCCCTCTCATCCTTTGACCAACCCTCCAACTCTGCTTCTCACCATCAGCTAAAGG GACCAGTCCCTAGGGGAGCCAAGAGCTCCCAAGGAAGGATGAAGGAGAGAAGGCAAGCATCCCAACCAGA TCCAAGGGGACACTGTCCCCATGCTGAATGCTCAGGACTCTTGGGTCCTGCCCTGTTTACGGATCCCTCA TTTTGTGTAATAAAGTGCATTGAGTGTCCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488528 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488528 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16967 same embryo
	EMAGE:16971 same embryo
	EMAGE:16969 same embryo
	EMAGE:16968 same embryo
	EMAGE:16966 same embryo
	EurExpress:euxassay_007233 same experiment
	MGI:4826014 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source