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EMAGE:16983

Lman1 lectin, mannose-binding, 1 ( MGI:1917611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983
"Pseudo-wholemount" of euxassay_008801. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008801_01 euxassay_008801_02 euxassay_008801_03 euxassay_008801_04
EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983
euxassay_008801_05 euxassay_008801_06 euxassay_008801_07 euxassay_008801_08 euxassay_008801_09
EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983
euxassay_008801_10 euxassay_008801_11 euxassay_008801_12 euxassay_008801_13 euxassay_008801_14
EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983 EMAGE:16983
euxassay_008801_15 euxassay_008801_16 euxassay_008801_17 euxassay_008801_18 euxassay_008801_19
EMAGE:16983 EMAGE:16983 EMAGE:16983
euxassay_008801_20 euxassay_008801_21 euxassay_008801_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16983Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16983_wholemount_strong.wlz
16983_wholemount_moderate.wlz
16983_wholemount_weak.wlz
16983_wholemount_possible.wlz
16983_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16983_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1243
Entity Detected:Lman1, lectin, mannose-binding, 1 ( MGI:1917611)
Sequence:sense strand is shown

>T1243
TCCTCGAGNCTGTTGGCCTACTGGAGATCCAAGATGGCGGTGTCCAGGCGGAGGGTTCCCCAGGCTGGAG
CGCGGTCGTTCTTCTGCGCCCTGCTGCTGTCTTTCAGCCAATTCACCGGGAGCGACGGCACGGGAGGCGG
CGCTGCGGCTCCCGGCGCCGCGGGCACCCAGGCCGAGTTGCCACACCGCCGTTTCGAGTACAAATACAGC
TTTAAGGGGCCGCATCTGGTGCAGAGCGATGGGACCGTGCCCTTTTGGGCCCACGCGGGGAATGCTATTC
CAAGTGCAGATCAGATTCGAATAGCGCCGTCATTAAAAAGCCAAAGAGGTTCCGTGTGGACCAAAGCAAA
AGCAGCCTTCGAGAACTGGGAAGTGGAAGTGACATTCCGAGTGACCGGAAGAGGTCGAATTGGAGCCGAT
GGCTTAGCAATTTGGTATACAGAAAATCAAGGCTTGGACGGCCCTGTGTTTGGATCAGCTGATACGTGGA
ATGGTGTTGGAATATTTTTTGATTCTTTTGACAATGATGGAAAGAAAAATAATCCTGCTATAGTAGTTAT
AGGCAACAATGGACAAATCAAT
Notes:The probe template was PCR amplified from IMAGE:2225161 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2225161 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16982 same embryo
 EMAGE:16980 same embryo
 EMAGE:16979 same embryo
 EMAGE:16978 same embryo
 EMAGE:16981 same embryo
 EurExpress:euxassay_008801 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS