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EMAGE:16993

Eif3e eukaryotic translation initiation factor 3, subunit E ( MGI:99257)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993
euxassay_008813_01 euxassay_008813_02 euxassay_008813_03 euxassay_008813_04 euxassay_008813_05
EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993
euxassay_008813_06 euxassay_008813_07 euxassay_008813_08 euxassay_008813_09 euxassay_008813_10
EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993
euxassay_008813_11 euxassay_008813_12 euxassay_008813_13 euxassay_008813_14 euxassay_008813_15
EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993 EMAGE:16993
euxassay_008813_16 euxassay_008813_17 euxassay_008813_18 euxassay_008813_19 euxassay_008813_20
EMAGE:16993 EMAGE:16993
euxassay_008813_21 euxassay_008813_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:16993Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
16993_wholemount_strong.wlz
16993_wholemount_moderate.wlz
16993_wholemount_weak.wlz
16993_wholemount_possible.wlz
16993_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:16993_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1309
Entity Detected:Eif3e, eukaryotic translation initiation factor 3, subunit E ( MGI:99257)
Sequence:sense strand is shown

>T1309
TCCTCGAGCCTGTTGGCCTACTGGGGCAAGATGGCGGAGTACGACCTGACTACTCGCATCGCGCATTTTC
TGGATCGGCACCTGGTCTTTCCGCTTCTTGAGTTTCTCTCTGTGAAAGAGATTTATAATGAAAAAGAATT
ATTACAAGGAAAATTAGATCTTCTTAGTGATACCAATATGGTGGACTTTGCTATGGATGTTTACAAAAAC
CTTTATTCTGATGATATCCCTCATGCTTTGAGAGAAAAAAGAACCACAGTTGTTGCGCAGCTGAAACAGC
TCCAGGCAGAAACAGAACCAATTGTGAAGATGTTTGAAGATCCAGAAACTACAAGGCAGATGCAGTCAAC
CAGGGATGGCAGGATGTTATTTGACTACCTGGCAGACAAACATGGGTTTAGGCAAGAGTACTTAGATACA
CTCTACAGATACGCAAAATTCCAGTATGAGTGTGGAAATTACTCTGGAGCTGCAGAGTATCTTTACTTCT
TTAGAGTTTTGGTCCCAGCAACAGATAGAAATGCTTTAAGTTCGCTCTGGGGAAAACTGGCCTCTGAAAT
CTTAATGCAG
Notes:The probe template was PCR amplified from IMAGE:2259135 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2259135 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:16994 same embryo
 EurExpress:euxassay_008813 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS