EMAGE mouse gene expression spatial database

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EMAGE:17003

Elf3 E74-like factor 3 ( MGI:1101781)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008831. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008831_01	euxassay_008831_02	euxassay_008831_03	euxassay_008831_04

euxassay_008831_05	euxassay_008831_06	euxassay_008831_07	euxassay_008831_08	euxassay_008831_09

euxassay_008831_10	euxassay_008831_11	euxassay_008831_12	euxassay_008831_13	euxassay_008831_14

euxassay_008831_15	euxassay_008831_16	euxassay_008831_17	euxassay_008831_18	euxassay_008831_19

euxassay_008831_20	euxassay_008831_21	euxassay_008831_22	euxassay_008831_23	euxassay_008831_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17003_wholemount_strong.wlz

17003_wholemount_moderate.wlz

17003_wholemount_weak.wlz

17003_wholemount_possible.wlz

17003_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17003_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

oral region

strong

regional

strong expression: see section 19

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

rectum

strong

regional

strong expression: see section 12

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3196
Entity Detected:	Elf3, E74-like factor 3 ( MGI:1101781)
Sequence:	sense strand is shown >T3196 GCGGAGCCTTCGACCGCTTAGATTTCTTCCCTTCCTGTGGCCTCAGAAGCCTGCTCACCCGCCTGCCACA CCGAACCCTGACACACCTCGGGGCCCTCATGGCTGCCACCTGTGAGATCAGCAACGTTTTTAGTAACTAC TTCAACGCCATGTACAGCTCAGAAGACCCCACCCTGGCTCCTGCTCCTCCGACTACCTTTGGCACTGAAG ACTTGGTGTTGACCCTGAACAACCAACAGATGACACTGGAAGGTCCAGAGAAGGCAAGCTGGACTAGCGA GCGGCCCCAGTTCTGGTCGAAGACCCAGGTTCTGGAGTGGATCAGCTACCAAGTGGAGAAGAACAAGTAT GACGCCAGCTCCATCGACTTCTCCCGCTGCGACATGGACGGAGCCACCCTCTGCAGCTGTGCGCTGGAGG AGCTGCGGCTAGTCTTTGGACCTCTGGGAGACCAGCTCCATGCCCAGCTTCGGGACCTCACCTCCAACTC TTCTGATGAA
Notes:	The probe template was PCR amplified from IMAGE:1548694 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1548694 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17004 same embryo
	EMAGE:17005 same embryo
	EurExpress:euxassay_008831 same experiment
	MGI:4824520 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source