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EMAGE:17018

Acly ATP citrate lyase ( MGI:103251)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018
"Pseudo-wholemount" of euxassay_008824. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008824_01 euxassay_008824_02 euxassay_008824_03 euxassay_008824_04
EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018
euxassay_008824_05 euxassay_008824_06 euxassay_008824_07 euxassay_008824_08 euxassay_008824_09
EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018
euxassay_008824_10 euxassay_008824_11 euxassay_008824_12 euxassay_008824_13 euxassay_008824_14
EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018
euxassay_008824_15 euxassay_008824_16 euxassay_008824_17 euxassay_008824_18 euxassay_008824_19
EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018 EMAGE:17018
euxassay_008824_20 euxassay_008824_21 euxassay_008824_22 euxassay_008824_23 euxassay_008824_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17018Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17018_wholemount_strong.wlz
17018_wholemount_moderate.wlz
17018_wholemount_weak.wlz
17018_wholemount_possible.wlz
17018_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17018_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
strong strong
regionalstrong expression: see section 07 08 09 10 16 17 18
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
facial vii ganglion
strong strong
regionalstrong expression: see section 03 04 18 19 20
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 06 17
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 07
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 05 06 17 18
trigeminal v nerve
strong strong
regionalstrong expression: see section 09 16
spinal cord
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 08 09 10 14 15
cervical ganglion
strong strong
regionalstrong expression: see section 07 08 15 16
thoracic ganglion
strong strong
regionalstrong expression: see section 11 13 14
dorsal root ganglion
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 15 16 17
neural retina
strong strong
regionalstrong expression: see section 01 02 03 04
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 15 16 17 18
lower jaw incisor
strong strong
regionalstrong expression: see section 10 11 12 13 14 15
lower jaw molar
strong strong
regionalstrong expression: see section 07 08 18
upper jaw incisor
strong strong
regionalstrong expression: see section 11 12 15 16
upper jaw molar
strong strong
regionalstrong expression: see section 07 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3188
Entity Detected:Acly, ATP citrate lyase ( MGI:103251)
Sequence:sense strand is shown

>T3188
TGGCCTCGAGCCAGATTCGGCACGAGGTTTTTTTTTTCTATGTTATGTGTTAAGGGTTTATTTAGTATCC
CACTGAAATGTTCCATGTTACAGACCAGTGTCTACTTATGTCAAGGGGAGGAGGGCTGGGACCATTGTAT
CCTTAGCCATTGTTGCACATGTGGTGTAGTAACTTAAATGTCAAGTTGTAACATACAAGTGTTTAAAATG
GAAACGCAAAGCAAAAAGCTGTGAAATGTCTTGTGTCAGTGTCCTCTGTGTTCATGCAGCCGACTTGTCT
GTTACTGAAGTGTGGGTCCAAAGACTCACAGCTGTTCTGCATCTGTAACCCACAAAGATTCTGGGCAGCT
GCCACCTCAGTCTCTTCTCTGTGTTATCTATCATGTTTGCTTTAAATAAACTAGATAGTAACAAAAAAAA
AAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1548573 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1548573 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17019 same embryo
 EurExpress:euxassay_008824 same experiment
 MGI:4822920 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS