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EMAGE:17025

Rtcd1 RNA terminal phosphate cyclase domain 1 ( MGI:1913618)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:17025 EMAGE:17025 EMAGE:17025 EMAGE:17025 EMAGE:17025
"Pseudo-wholemount" of euxassay_008846. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008846_01 euxassay_008846_02 euxassay_008846_03 euxassay_008846_04
EMAGE:17025 EMAGE:17025 EMAGE:17025 EMAGE:17025 EMAGE:17025
euxassay_008846_05 euxassay_008846_06 euxassay_008846_07 euxassay_008846_08 euxassay_008846_09
EMAGE:17025 EMAGE:17025 EMAGE:17025 EMAGE:17025 EMAGE:17025
euxassay_008846_10 euxassay_008846_11 euxassay_008846_12 euxassay_008846_13 euxassay_008846_14
EMAGE:17025 EMAGE:17025 EMAGE:17025
euxassay_008846_15 euxassay_008846_16 euxassay_008846_17

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:17025Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
17025_wholemount_strong.wlz
17025_wholemount_moderate.wlz
17025_wholemount_weak.wlz
17025_wholemount_possible.wlz
17025_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:17025_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3210
Entity Detected:Rtcd1, RNA terminal phosphate cyclase domain 1 ( MGI:1913618)
Sequence:sense strand is shown

>T3210
GGTTGCCGGTGTTTTGCCACTAAAAGTAGCAAAAGATATGGCGGCGGCGGCTGTGCGGTGCATCCGGAAG
GAGATCCGGGATCTGTACGTCAGCATCCAGCCCGTGCAGGAGGCCAGGGACCAAGCCTTCGGCAATGGGA
GTGGAATCATCATTGTTGCTGAGACATCCACTGGCTGCTTGTTTGCTGGATCATCACTTGGCAAAAGAGG
TGTGAATGCAGACAAGGTTGGGATTGAAGCTGCTGAAATGCTATTAGCGAACCTTAGACACGGTGGCACT
GTGGATGAGTATCTACAAGACCAGCTGATTATTTTCATGGCGCTAGCCAATGGAATTTCCAGAATAAAAA
CAGGATCAGTCACACTCCACACGCAGACTGCTATACATTTTGCTGAACAGCTAGCAAAGGCCAAATTTAC
CGTGAAGAAATCAGAAGAGGAAGAAGATGCCACTAAGGACACTTACGTCATCGAGTGTGAAGGAATTGGG
ATGGCAAATCCACACCTCTAGGGTGTTCACGTACGCTCTACCTCCT
Notes:The probe template was PCR amplified from IMAGE:1617648 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1617648 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17023 same embryo
 EMAGE:17026 same embryo
 EMAGE:17024 same embryo
 EMAGE:17022 same embryo
 EurExpress:euxassay_008846 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS