EMAGE mouse gene expression spatial database

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EMAGE:17029

Elavl1 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) ( MGI:1100851)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008842. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008842_01	euxassay_008842_02	euxassay_008842_03	euxassay_008842_04

euxassay_008842_05	euxassay_008842_06	euxassay_008842_07	euxassay_008842_08	euxassay_008842_09

euxassay_008842_10	euxassay_008842_11	euxassay_008842_12	euxassay_008842_13	euxassay_008842_14

euxassay_008842_15	euxassay_008842_16	euxassay_008842_17	euxassay_008842_18	euxassay_008842_19

euxassay_008842_20	euxassay_008842_21	euxassay_008842_22	euxassay_008842_23	euxassay_008842_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17029_wholemount_strong.wlz

17029_wholemount_moderate.wlz

17029_wholemount_weak.wlz

17029_wholemount_possible.wlz

17029_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17029_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3215
Entity Detected:	Elavl1, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) ( MGI:1100851)
Sequence:	sense strand is shown >T3215 ANNCCTGTTTGTTATCGAGGCCGCCTTTCTCCAGTGCCTGTGCCCCCTGCCCACTCAGTCCCAGCTCTAT AGTGTTGGGAGCAGCAGCAGGGCCCATATTCTGGGGCTCCCACAAGGGGAGATGGGAAGAGGTTCCACAT GTTGGGCTGAGCCAGGCCCTCCAGAGACACAAAAGGTGTTTTGTAAGCCCAGGCACCAATGAGAACGGAC CAAAGAGTTTCAGGGCAGCTCCAGTATATTCCAGAGTCAAACCTGAGCTCCAGGCATGCCTGAAGATGCC CCACTCCTCTGACATCCTGAGCGTCTGTCCACACATTGCATGCATGGTGCCCACACATCAGATACTGTTG TTCATGCAACTTCCCGAGTTTCCAAGACCATTTAACCTAGCTTGAATGCACAACGATTGCTCTGTTTTTA ATGACACAGAAACATTTGAGCATTGTATTTTCTCGCATCCCTTCTTGTGAGCACTAGACTTTTTCCAATC
Notes:	The probe template was PCR amplified from IMAGE:2698770 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2698770 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17028 same embryo
	EMAGE:17027 same embryo
	EurExpress:euxassay_008842 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source