EMAGE mouse gene expression spatial database

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EMAGE:17037

Olig1 oligodendrocyte transcription factor 1 ( MGI:1355334)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007237. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007237_01	euxassay_007237_02	euxassay_007237_03	euxassay_007237_04

euxassay_007237_05	euxassay_007237_06	euxassay_007237_07	euxassay_007237_08	euxassay_007237_09

euxassay_007237_10	euxassay_007237_11	euxassay_007237_12	euxassay_007237_13	euxassay_007237_14

euxassay_007237_15	euxassay_007237_16	euxassay_007237_17	euxassay_007237_18	euxassay_007237_19

euxassay_007237_20	euxassay_007237_21	euxassay_007237_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17037_wholemount_strong.wlz

17037_wholemount_moderate.wlz

17037_wholemount_weak.wlz

17037_wholemount_possible.wlz

17037_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17037_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

strong

single cell

strong expression: see section 09 10 11 12 13 14 15 16 17

diencephalon lateral wall mantle layer

strong

single cell

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

single cell

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 20 21 22

telencephalon marginal layer

strong

single cell

strong expression: see section 19

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

pons mantle layer

strong

single cell

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

pons ventricular layer

strong

single cell

strong expression: see section 05 06 07 16 17 18

midbrain mantle layer

strong

single cell

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord mantle layer

strong

single cell

strong expression: see section 06 07 08 09 10 11

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 15 16 17 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3961
Entity Detected:	Olig1, oligodendrocyte transcription factor 1 ( MGI:1355334)
Sequence:	sense strand is shown >T3961 TGCCGACCGGCCCACCCCTGGGCGTTAGTGAAGGGCGCCCCGGGTCGCCCCACAGCCCCCGCCCCAGATG TACTATGCGATTTCCCAGGCGCGTGTGAACGCGGCTCCCGCGACCATGTTGCGGCCACAGCGGCCTGGAG ACGTGCAGCTCGGGGCCTCGCTGTATGAGCTGGTGGGTTACAGGCAGCCACCTATCTCCTCATCCTCATC CTCATCCTCTTCCACCGCATCCCTTCTCCCCAAGCCTGCGCGCGAGAAGGCCGAAGCTCCGCTCGCTGAA CCTCGGGGACCTGCGCCAGAGTCCGGGGGCGCCCGGGCCGACGCCAAAGAGGAACAGCAGCAGCAGCAGC TGCGGCGCAAGATCAACAGCCGCGAGCGGAAGCGCATGCAGGACCTGAACTTGGCCATGGACGCGCTGCG CGAAGTTATCCTACCCTACTCGGCAGCGCACTGCCAGGGCGCGCCGGGCCGCAAGCTCTCCAAGATTGCC ACGCTGCTGCTCGCCCGCAACTACATCCTGCTGCTGGGTAGCTCGCTGCAGGAGCTGCGCCGCGCGCTCG GCGACGGTGCGGGACCCGCCGCCCCGCGCCTGCTCCTCGCTGGCCTGCCTCTTCTGGCTGCCGCGCCGGG CTCTGTGCTGCTGGCACCCGGTGCCGTGGGACCCCCCGAAACGCTGCGCCCCACCAAGTACCTGTCTCTA GCGCTCGACGAGCCACCGTGCGGCCAGTTCTCTCTCCCAGCTGGTGGCGCGGGTAGCCCTGGCCTCTGCT CCTGTGCGGTGTGCAAGTTCCCGCATCTGGTCCCCGCCGGCCTGGGCTTGGCAGCGGTGCAGGCCCAGTT CTCCAAGTGAGGGCTGGCTGGGCCCGGGGCGGAGCGCGACCTCAGCCAATCTTCCCGCGCCCCCGCGTCC TGGATCTAGGCAGAGCTGGCCCCAGAAAGGAGGACATTTCCAGACTTCTCTCCCAGACGACAGACTGTAG GGCGCCTTCTTCCCCGCCCCCACTCCCTGGCAATTAAAATAACCCAAGCGGGTGTTCCAAGGAGCGATGT AGTTGCTTGGGATTCTAGATGAATCCCACCTGTTTAGAGCCAGAAGTCTTCGCATCCCCACCACAACTCA CCCACTGGCAGGGGTGACAACCCCCCACCACCGCGGGCTATTCTCGTATCCGTGAGCATCGCGGAGTCGG GGAGGACGGTAGCCGGCCGGTGCGGTATAAGGGGGTTGCCCTGGGTGCAGCGAAAGGCTGCCCCCAGCTT GGTTTCCTGGCATGCTTCCGCTCCCTGGCCTAAGAGCTGCTCCCCAACAGTGTCTACCCAGAGGGGCGTC CTTTCCTTGTCCAGGGCCTGAGCGCACAGGCTCCCTTGCTCTCTCCAGCCAAACCATGCGGATCTAGGAG GTCTAGAAAGCCCAGGCCACCGGTACAAACATCAAGGGTGTTACGGATGGGCCGCTCTGGAAAGCCCAGG GTTTCCGAGCTGGATGTTACGCGGGTGCGCTCGGTTACCGCGCTCGGTGGTTGGGGATGCGAAGTGGAGA GGTTCCTCTCCACTCCCAGTTCTGTTTTTCAGGCTCGCCCAGGTGTTTTGTTGCAGTTTATGCTCCTTAA AACCTAAAACCCTTACTTAGTCTCCACTTCCCTAAAGGTAGCTTAACCAACGTTTGAGCTTGCTTGAAAA CAAAATTCCAACCACGTTCCATGCCATCGGTGTTCGGACTTACTAAAATAGGTAGCAAGGCACGTCGTAG CGCAGGCTTATAAAATTCGGTCATTAACGTTCTCATTAAAGTAAGCGGAGTTGGGCGAGGATCCCAAACC GCCTACCCGCCGCGCTGCAGGCGGGAAGGCGCTGGCACTTCACAGGCTGGTCCTTAACCACAGCAGTGCG GCTGAAGAACTCCGTGGACGCGTGCGCGCGTGGGTGCCGCGTCTTGGCTTGTGACTAGCGTTAGGAAATT ACCCAAGATTATTGCATAATCTTCACCAGCTTGCTTTGATTCTTTTTTATGTTGGAAGTTTTGGGTTGTT GACAGTAGCCGAATTTAACTGGCATTTTATTTGACCTCTAACTCTGTCCCTCCTGAACTGTACAGAAATC ACAAAATAAAACGTCAACAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6314266 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6314266 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17039 same embryo
	EMAGE:17038 same embryo
	EurExpress:euxassay_007237 same experiment
	MGI:4826967 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source