EMAGE mouse gene expression spatial database

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EMAGE:17083

Rcor1 REST corepressor 1 ( MGI:106340)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011825_01	euxassay_011825_02	euxassay_011825_03	euxassay_011825_04	euxassay_011825_05

euxassay_011825_06	euxassay_011825_07	euxassay_011825_08	euxassay_011825_09	euxassay_011825_10

euxassay_011825_11	euxassay_011825_12	euxassay_011825_13	euxassay_011825_14	euxassay_011825_15

euxassay_011825_16	euxassay_011825_17	euxassay_011825_18	euxassay_011825_19	euxassay_011825_20

euxassay_011825_21	euxassay_011825_22	euxassay_011825_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17083_wholemount_strong.wlz

17083_wholemount_moderate.wlz

17083_wholemount_weak.wlz

17083_wholemount_possible.wlz

17083_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17083_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17

vomeronasal organ

strong

regional

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4455
Entity Detected:	Rcor1, REST corepressor 1 ( MGI:106340)
Sequence:	sense strand is shown >T4455 ATAAGTTTGGCGGCGGCGGCTCCCAACGGGAACAGCGGCAGCAACTCCTGGGAGGAAGGCAGCTCGGGCT CGTCCAGCGACGAGGAGCACGGTGGCGGTGGCATGCGGGTGGGACCCCAGTACCAAGCGGCGGTGCCCGA CTTCGATCCTGCCAAACTGGCAAGGCGCAGTCAAGAACGAGACAATCTTGGCATGTTGGTCTGGTCACCT AATCAGAGTCTTTCTGAAGCAAAACTGGACGAGTACATCGCTATCGCCAAAGAGAAGCACGGGTACAACA TGGAGCAGGCTCTTGGGATGCTCTTTTGGCATAAGCATAATATTGAAAAATCATTGGCCGACTTGCCCAA CTTCACCCCCTTTCCAGATGAGTGGACTGTAGAAGACAAAGTCTTATTTGAGCAAGCCTTTAGTTTTCAT GGGAAAACTTTTCATAGGATCCAGCAAATGCTTCCTGATAAATCCATAGCAAGTTTGGTGAAGTTTTACT ACTCTTGGAAGAAAACTAGAACTAAAACAAGTGTGATGGATCGCCATGCTCGGAAACAGAAGCGGGAGCG GGAGGAAAGTGAGGATGAACTGGAAGAAACAAACGGCAGTAATCCCGTTGACATTGAGATTGACCCAAAC AAGGAAAGCAAAAAGGAGGTGCCCCCTACAGAGACAGTTCCTCAGGTCAAAAAAGAAAAGCACAGCACAC AAGCTAAAAATAGAGCAAAAAGGAAACCTCCGAAAGGCATGTTTCTTTCTCAAGAAGATGTGGAGGCTGT GTCTGCTAATGCTACTGCTGCGACCACGGTGCTGAGACAGCTGGACATGGAGCTGGTCTCCATCAAACGA CAGATTCAGAACATCAAGCAGACGAACAGTGCTCTTAAAGAGAAGCTTGATGGTGGGATAGAGCCTTACC GACTCCCAGAGGTTATTCAAAAATGCAACGCTCGCTGGACAACGGAAGAGCAGCTTCTCGCCGTACAAGC CATTAGGAAATATGGGCGAGATTTTCAGGCGATCTCAGATGTGATTGGGAACAAGTCAGTTGTTCAGGTG AAAAACTTTTTTGTAAATTATCGACGCCGCTTCAACATAGATGAAGTTTTACAAGAATGGGAAGCAGAAC ATGGGAAAGACGAGACCAATGGACCTGCTAACCAGAAACCTGTCAAGTCCCCAGAAAGCTCCATCAAGAT CCCGGAGGAGGAAGATGAGGCTGCTTCTGTCCTCGACGTAAGATACGCATCTGCCTCATGAAAACTGTTG GTAGCTTTGAACACTTGGTATGGACGACACGTTATCCAGGTATCAGGTATTACAAGACATCACCTAGCCA TCTGCATCACATCTCTGTGGACAAGCAGCTATTACCAAAAAAGGCATACACTTCCAGTCCCGTGCTACAT CTGCCTTAATTCTTTGCTCGTTCCTCCATGTTGGCACGACTTCCCAGAGAGCTCCGTTGCATCTCATTCT GCCTAAGGGCTGGGGGACCCACGGCCTGCACACCTCCCTCGAGTCTGGGACCTACCCTCCAGTTGGCTCC CCCCCGAGCCCCAACAATGGCAGCTCTCCCAATCAACAGCTTCAGAACAGGTAGTCTTGAAAGGCCCAGC TCTGTTCCTGCATGGCCTGCAGTTTCTACTTTGTGCATAGTGTAATTCTCAGAAGGCCTAGTAGACCTTC TAGAAAAAGTCTCCCCTTCTCTCCTGGGGCAGCCACCTAAGTAAAATCATGCTTCTTTGGAAATCACAAG CCCTTCAGCAGACCACATCGTCACCTTTATCATACAAGCTTCCTGATACAACTCCTGTTTCCCTAAGAGA GGACAGTTATGGCTGTAGATCAGGTGGCTTCTCTCCCCTTTCTCTCTCCCCTAACTCTTTCCCTTTTCTC CTTTCCAAGTGGTAAAAGCTCATTTGCAGCAACTGTCCTTGGGCACTGTAGCCTCATGGGAACGTGGACC TCAGTGCTTTCTGCCTTCAGCCTTCAGCATTGTAATCCCAGGATGAGGGTGCACCCATCTCTTCTTGATC CCCAGCTGCCACCATCATCCCCAGCTCCGAGAGATTTGGTTCCTATCATTGTACCTGGTGCTTGTACATT TGGAATTGGTGCCTTCTCCTTCTGGCAACCATGTTATCAATCCCTTTTCTGTTTAAGTGTCTTACTTAAA GTTTATTGCTTGCCAAAGATGCCATCATTGAGGTGTAGCAGATGGGGAGGGTGTCTGCAGGTTTGAGTGC AGACTTTAAATGTCAGGTCAGCTCAGGAAAGGAGAAGAGAGAAGCTAGGGATGGTTTCCTAGACGCTGCA CACCAGTAGCTCTCATCCTCTTGGCGTGCCTGGCCATTGTATGTCACTCCATGTGTCCTTTTGTGTCATT TGTGTACTGGTGTGATCACACAAACACTATATCCTGTCTCTTAGCAGCAGATAGGACAGATAAGTAATGG ATGTGTTTGTTGCTTAGTGAGACCAAGATGAAAAGAAACAACCTAGAGTGGTGGCAATGCCATTTGTTTG GACTGGGAACAAGCAAGGGTTGCTAATAAATGTTCTGAGACTTCAGAATCACTTTTGCTCTCTTACCAGA CTGTGGGCCTGAAGGGCCTGAGGAGCAGCCGGCTGGGCTGTGTCCTAGCATCCATGGCCACTTGAGACTG CACTAACCATGTGGAAATATCTTTTGTTGTTGTTTTAATATTTTTTCTGCTTTCAATACCAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4936863 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4936863 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17081 same embryo
	EMAGE:17082 same embryo
	EMAGE:17080 same embryo
	EMAGE:17085 same embryo
	EMAGE:17084 same embryo
	EurExpress:euxassay_011825 same experiment
	MGI:4827668 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source