EMAGE mouse gene expression spatial database

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EMAGE:17085

Ddx47 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 ( MGI:1915005)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001477. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001477_01	euxassay_001477_02	euxassay_001477_03	euxassay_001477_04

euxassay_001477_05	euxassay_001477_06	euxassay_001477_07	euxassay_001477_08	euxassay_001477_09

euxassay_001477_10	euxassay_001477_11	euxassay_001477_12	euxassay_001477_13	euxassay_001477_14

euxassay_001477_15	euxassay_001477_16	euxassay_001477_17	euxassay_001477_18	euxassay_001477_19

euxassay_001477_20	euxassay_001477_21	euxassay_001477_22	euxassay_001477_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17085_wholemount_strong.wlz

17085_wholemount_moderate.wlz

17085_wholemount_weak.wlz

17085_wholemount_possible.wlz

17085_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17085_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4454
Entity Detected:	Ddx47, DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 ( MGI:1915005)
Sequence:	sense strand is shown >T4454 GAGCAATATGGCGGCGGACGAGGAACCCGATTCCCCCTCTGGAGCCCTTCAGACAGCTGCGGAAGAGGAG GAAACGAAAACATTTAAAGACCTGGGTGTGACGGATGTATTGTGTGAAGCTTGTGACCAGTTGGGATGGG CAAAGCCCACCAAGATCCAGATCGAAGCTATTCCTTTGGCCTTGCAAGGTCGTGATATTATTGGGTTGGC AGAAACGGGATCTGGGAAAACAGGTGCCTTTGCTTTGCCCATCCTGAATGCCCTGCTGGAGACGCCCCAG CGACTGTTCGCCCTGGTCCTCACCCCTACTCGAGAGTTGGCCTTTCAGATCTCTGAGCAGTTCGAGGCCC TGGGGTCATCTATTGGAGTGCAGTGTGCTGTCATTGTTGGTGGAATTGATTCAATGTCTCAGTCACTAGC ATTGGCCAAAAAACCACATATAGTAATAGCTACTCCTGGCCGACTGATTGACCACTTGGAAAATACCAAA GGCTTCAACTTAAGAGCTCTTAAGTACTTGGTCATGGATGAAGCAGACCGGATACTGAACATGGATTTTG AGACAGAGGTTGACAAGATCCTCAAAGTGATTCCCCGAGATCGGAAAACATTTCTCTTTTCTGCCACCAT GACCAAGAAGGTACAAAAACTTCAGCGTGCAGCTCTTAAGAACCCTGTGAAATGTGCTGTGTCCTCTAAG TACCAGACTGTGGAGAAACTGCAGCAGTATTACCTTTTTATTCCCTCGAAATTCAAGGATACCTACCTGG TTTATATTCTCAATGAACTGGCTGGAAACTCCTTTATGATATTCTGCAGCACCTGTAACAACACTCAGAG AACAGCTTTGCTGCTCCGAAATCTCGGGTTCACTGCCATCCCCCTCCATGGACAGATGAGTCAGAGCAAG CGCCTCGGATCCCTTAATAAGTTTAAGGCTAAGGCGAGGTCCATTCTTCTAGCGACTGATGTCGCCAGCA GAGGTCTGGACATACCCCATGTGGATGTAGTGGTCAATTTTGACATTCCGACTCATTCTAAGGATTATAT CCATCGAGTAGGACGAACCGCGAGAGCTGGGCGCTCTGGGAAGGCCATTACTTTTGTAACACAGTATGAC GTGGAACTCTTCCAGCGCATCGAGCACTTGATTGGAAAGAAGCTGCCTGTCTTTCCGACGCAGGATGAGG AGGTCATGATGCTCACGGAACGTGTGAACGAGGCCCAGAGGTTTGCCCGGATGGAGTTAAGGGAGCACGG AGAAAAGAAGAAACGGAAGCGAGAGGATGCAGGAGATGATGATGATAAGGAGGGCGCCATTGGTGTGAGG AACAAGGTGGCTGGGGGCAAAATGAAGAAGCGGAAAGGCCGCTAATGCTTTAGAAAGACTTCTGCTCGCC TGCTCGCCAGCTGTGAGATTCTGTCCAGAGACCACTCGGCCAGCTGGGAGCACGTTCCTCGGGATTGCAG TGTCATTGCAATGGGACAACTTTTACAGTGTTGACACAGGACTGGCCGCTCTTCAGCTTGCATTCCTCAC CTGTGACACAAGTGCCGTGTGCTCTTCATGCTGCACCTCTGCCTTCCCACCTGCAAGCCACGGCCTGGCG GATGGAGAGCGCTGACTGTAGTTAACTGTAAAGCTTCTACAGAATGGTGTGTACGCCACCTTATCTCAGC CGGCATTCAGCCTGATGTGGGTCAATAATTATTATGATTAAAGATGTATTTTTATTACCTGAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935970 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935970 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17081 same embryo
	EMAGE:17082 same embryo
	EMAGE:17080 same embryo
	EMAGE:17084 same embryo
	EMAGE:17083 same embryo
	EurExpress:euxassay_001477 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source