EMAGE mouse gene expression spatial database

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EMAGE:17120

Plcd3 phospholipase C, delta 3 ( MGI:107451)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001511_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17120_wholemount_strong.wlz

17120_wholemount_moderate.wlz

17120_wholemount_weak.wlz

17120_wholemount_possible.wlz

17120_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17120_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

moderate

regional

moderate expression: see section 01

lateral ventricle choroid plexus

weak

regional

weak expression: see section 01

medulla oblongata alar plate

weak

regional

weak expression: see section 01

lower jaw incisor

moderate

regional

moderate expression: see section 01

oral epithelium

moderate

regional

moderate expression: see section 01

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4499
Entity Detected:	Plcd3, phospholipase C, delta 3 ( MGI:107451)
Sequence:	sense strand is shown >T4499 GCCGCGGGCTGTGTTCCCGTTGCCCGGTTCTCACGCACAGATCCCGGGCCTGGCCCTGGGCCCTTCGAGA CCATGCTGTGCGGTGGCTGGAAAAGGAGTCGCCGCTCGCCGGAGGAATCCCGGGTGTCCGCCCAGGTCGC AGCGCCCCTCGCGTTCCCGCCCTCGCCCGCGTCGTCGGACAGCAGCACCAAAAGGCCTGGGCTACGGGCA CTGAAGAAGATGGGCCTGACGGAGGATGAGGATGTCCAAGCCATGCTGCGGGGCTCCCGACTCCTCAAGA TCCGCTCACGCACGTGGCACAAGGAGCGGCTGTACCGGCTGCAGGAGGACGGCCTGAGCGTGTGGTTTCA GCGGCGCATCCCTCGCGCAGCTTCTAAGCACATATTCTTCGTGCAGCACATCGAGGCTGTCCGGGAGGGT CATCAGTCGGAGGGCCTGCGGCGCTTCGGGGGCGCCTTCGCGCCAGCGTGCTGCCTCACCATCGCCTTCA AGGGCCGCCGCAAGAACCTGGACCTGGCAGCGCCCACTGCGGAGGAGGCACAGCGCTGGGTGCGAGGACT GGCCAAGCTGCGGGCGCGCCTGGATGCCATGAGCCAGCGGGAGCGCCTAGACCATTGGATCCATTCCTAT CTGCACCGGGCCGACTCCGACCAAGACAGTAAGATGAGCTTCAAGGAGATCAAGAGCCTGCTGAGGATGG TGAACGTGGACATGAACGACATGTACGCTTACCGCCTCTTCAAGGAGTGTGACCATTCCAACAACGAGCG TCTAGAGGGGGCCGAGATAGAGGCGTTCCTGCGGAGGCTGCTAAAGCGGCCTGAGCTGGAGGAGATCTTC CGCCGGTACTCCGGTGAGGACCGGGTGCTGAGTGCCTCTGAGCTGCTGGAGTTCCTGGAAGACCAGGGCG AGGATGGTGCCACCCTGGCCTGTGCCCAGCAGCTTATCCAGACTTATGAGCTCAATGAGACAGCCAAGCA GCACGAACTGATGACGCTGGATGGCTTCATGATGTATCTGTTGTCGCCTGAGGGGGCCGCCCTGAACGTG GCCCACACGTGTGTGTTCCAGGACATGGGTCAGCCCCTCGCCCACTACTTTATCTCGTCCTCTCACAATA CCTACCTGACGGACTCTCAGATCGGAGGGACCAGCAGCACAGAGGCTTATATTAGGGCCTTTGCCCAGGG ATGCCGCTGTGTGGAACTTGACTGCTGGGAGGGGCCGGGAGGGGAACCTGTCATCTACCATGGCCACACG CTCACCTCCAAGATCCTCTTTCGAGATGTGATCCAGGCTGTGCGTGACCACGCTTTCACGTCGTCCCCAT ATCCTGTAATCCTATCCCTCGAGAACCACTGCGGGCTGGAACAGCAGGCTGTCATGGCTCGCCACCTTCG AAGCATCCTGGGAGACATGCTGGTGACACAGGCCCTGGACTCCCAGAACCCCGAGGAGCTGCCATCTCCT GAGCAGCTCAAGGGCCGGATCTTGGTGAAGGGGAAGAAGCTGCCAGCTGCTCGGAGTGAGGATGGTCGAA TTCTGTCAGACAGGGAGGAGGAAGAGGAGGAGGAAGAGGAGGCGGAAGAGGCCTTGGAAGCTGCAGAGCA GAGGAGTCGAGCCAAGCAGATCTCCCCAGAGCTCTCAGCGCTGGCTGTGTATTGCTGTGCTACCCGCCTG CGGACCCTGGAACCCAGTCCTGGCCCGCCTCAGTCTTGCACGGTTGGCTCCCTCAGCGAGCGCAAGGCCA GGAAGTTCACCAGGGAGGCAGGGACCAGCTTTGTCAGGCACAACACTCAGCAACTGACCCGAGTGTACCC ACTGGGGCTGCGGATGAACTCAGCCAACTATAACCCCCAGGAGATGTGGAACTCCGGCTGTCAGCTGGTG GCTCTGAACTTTCAGACTCCTGGTTATGAGATGGACCTCAACACAGGGCGCTTCCTCATAAACGGGCAAT GTGGCTACGTCCTCAAACCTGCTTACCTCAGGCAACTCAACACCACCTTTGACCCTGAGTGCCCTGGACC CCCCAGAACTACTCTGGCAATCCAGGTGTTAACGGCCCAGCAGCTACCCAAGCTGAACGCAGAGAAGCCC AGCTCCATTGTGGATCCTCTGGTGCGCGTGGAGATCCACGGGGTGCCCGAAGACTGTGCCCAGAAGGAGA CAGACTACGTCCTCAACAATGGCTTCAACCCCTGCTGGGAGCAGACTCTGCAGTTCCGACTGCGGGCCCC AGAGCTGGTGCTGGTCAGGTTTGTGGTAGAAGATTATGACACCACCTCTCCCAATGATTTTGTGGGCCAG TCTACACTGCCTCTCAGCAGCCTGAAGCAAGGGTACCGCCACATCCACCTGCTGTCCAAGGATGGAGCCT CGCTCGCTCCAGCCACGCTCTTCGTGCACATCCGCATTCAGAACTCTTGAGGGCGTCACTGACTTAACCC TAACATCAGCAGGGCTGGCGTCGTGGCACGGAGCCCTCTCTGGAGCACAAGACCATGGGAGTCCCAGCCC CTCACACCAGGTCCCTGTCTGGGTCACCAGGTGCTGTGCACACCTGGGTGCTAGGAGCTGTTTGGTTGCT CAGTGAGAGAGAGGACCATGATTGGTGTCCTGACCAGTGGATTTCAGGGGCCTCCAACCTTGGGGCTCTG AGGTGGTCTCAGATTCCCTGGTCAACTTGAGGAGCAGGACACGTGTCCTTCTGCCCTCCAGCGGGGCCAT CGTAACCTTCATCCCTGAGAAGGGCACAGCCACCTTCACTGCCTCCTTTCAGGGTGAGCAGAACCCCAGA GCCTCCTTCCCCTTAGAAGGGACTTTTCTGGCTTCTCCTTCCCTTTCTGTCTCAGGCAGGTGGGGAGCAG CTGAGCCCTGGGAGAAACCTGGCAGTAGGTTTGTAACTTGTGTTATAGAAGAGCCCAAGAGGGCAGAAGA GTAACTATTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4952407 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4952407 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17118 same embryo
	EMAGE:17117 same embryo
	EMAGE:17119 same embryo
	EurExpress:euxassay_001511 same experiment
	MGI:4827253 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source