EMAGE mouse gene expression spatial database

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EMAGE:17155

Cndp1 carnosine dipeptidase 1 (metallopeptidase M20 family) ( MGI:2451097)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001542. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001542_01	euxassay_001542_02	euxassay_001542_03	euxassay_001542_04

euxassay_001542_05	euxassay_001542_06	euxassay_001542_07	euxassay_001542_08	euxassay_001542_09

euxassay_001542_10	euxassay_001542_11	euxassay_001542_12	euxassay_001542_13	euxassay_001542_14

euxassay_001542_15	euxassay_001542_16	euxassay_001542_17	euxassay_001542_18	euxassay_001542_19

euxassay_001542_20	euxassay_001542_21	euxassay_001542_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17155_wholemount_strong.wlz

17155_wholemount_moderate.wlz

17155_wholemount_weak.wlz

17155_wholemount_possible.wlz

17155_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17155_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

kidney calyx

moderate

regional

moderate expression: see section 04 05 06 07 08 14 15 16 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4745
Entity Detected:	Cndp1, carnosine dipeptidase 1 (metallopeptidase M20 family) ( MGI:2451097)
Sequence:	sense strand is shown >T4745 GTCACCGTGAAAAACCAAGTTAATAAAACACAATTTTCCCCCCTGTCATAATAAATTCATTGAAGCAGAA ACAGGACAGAGCAGCCTTCACCACACAAGAACATTAGGACGGCGTGAGACTTGGCTTCGTCTCTCTCGGC ACTGCTGCTGTGGGCCAATCACAGGCTTCGTCTCTCTCGGCACTACAGCTGGAGCAGGCAATGTTCTCTT CGGCACACTCTGGGCTGTTGGAGAAGCTCTTCCACTACATAGACCTCCACCAGGATGAGTTCGTGCAGAC ACTGAAGGAGTGGGTGGCCATTGAGAGTGATTCTGTGCAGCCTGTGCCTCGTCTCAGACAGAAGCTCTTC CAGATGATGGCCTTGGCTGCAGACAAACTCCGGAACCTGGGAGCTGGGGTGGAGTCCATAGACTTGGGTT CTCAGCAGATGCCTGATGGTCAGAGTCTCCCCATACCTCCTATCCTATTGGCTGAGCTGGGAAGTGATCC CGAGAAGCCAACTGTATGCTTCTATGGGCACCTGGACGTGCAGCCAGCTCAGAAGGACGACGGGTGGCTC ACGGACCCCTACACACTGACAGAGGTGGATGGGAAACTGTATGGACGGGGAGCAACAGACAACAAGGGCC CCGTCTTGGCTTGGATCAATGCCGTGAGCACCTTCAGAGCTCTGCAGCAGGATCTTCCCGTGAACATCAA ACTCATCCTGGAGGGAATGGAAGAAGCCGGTTCCATCGCCCTGGAGGAACTCGTCATGAGAGAAAAGGAC CACTTCTTCTCCAGTGTGGACTACATTGTGATTTCAGACAACCTGTGGCTCAGCCAGAGGAAACCTGCAC TCACTTATGGAACCCGGGGGAACTGCTACTTCACGGTGGAGGTGAAGTGTAGAGATCAAGATTTCCACTC AGGAACCTTTGGTGGCATCCTCAACGAACCAATGGCTGACTTGGTTGCTCTTCTAGGTAGCCTGGTGGAT TCATCAGGCCACATCCTAATTCCTGGAATCTATGATCAAATGGCACCTATTACAGAGGGAGAAAAGACAA TGTATAAAAACATTGATATGGACCTAGAAGAATACCAGAACATCAACCAAGTTGAGAAATTTCTGTTTGA TACCAAGGAGGAACTTCTGATGCACCTGTGGAGGTATCCGTCTCTCTCCATCCATGGCATCGAGGGTGCC TTTGATGAGCCTGGAACTAAAACAGTCATTCCCGGCCGAGTCCTAGGGAAATTTTCAATCCGTCTAGTCC CTACCATGTCTCCGTCTGTGGTGGAGAAACAGGTAACTCAGCATCTCGAAGCTGTCTTCTCTAAAAGGAA TAGTTTCAACAAGATGGCCGTTTCTATGGTCCTAGGACTCCACCCATGGACTGCAAACGTCAATGATACT CAGTACCTTGCAGCACAGAGAACCATCAAAACAGTGTTTGGAGTTAATCCTGACATGATCCGGGATGGGT CAACCATTCCAATTGCCAAAATATTCCAGGCTATCACACAAAAGAGTGTGATGATGCTCCCACTGGGAGC TGTGGACGATGGAGAGCACTCTCAGAATGAGAAGATCAACAGGTGGAACTACATACAGGGGTCGAAATTA TTCGCTGCCTTTTTCCTGGAGTTGTCAAAACAGCATTCAGGACACCAGATGCCTTCCAGTGTCTACTGAT CCACGGGCAGCACTCCCACCTCAGACGCTGGACAAAGCAGGAGTTGGGAAAATTCAGGAAATATGGAAAA TGAAAGGTTTCGAAGGCACAGACAGATATTGGAGATGTTTTAACAGTCCCTCCGTCACTTAACTTCCACA AGTCACAGCTACCGAGACCGTTAGTTCCTGAATCTACTGCAACAGGCTGGGGTAACCTTCTAATACAGGT GGGGTGTCTCTTTAGTCAATTAGATTCTCACTTTCAATTCCATTGGCTTAACACTCCAGTTCACTTTCCA GTCCCAAGAACTGTGGTTCCCAATCATCGGACCCAGGGTCTCAGGACCCGAGTCATCTTCACCTCTGTTC TCTTAATAATGGAAGTGTGGCTTCAACAACCGGCTACAGTGATTTCCTTGTGGATTCACTTTTAGAAGAG TTACTAGGGCATGTCTCTGTAATAACTTCTTGCCTCTCCTTCATTTGTTCCCCGACACACACCCCCCCCC CCGGATTCCTGAACACTCTGAAGCTAAATGATCAATATTCATAGCCTCACACCATTTGGAATCCTTGTGC AAAGATGATTTTTTTCTTTAATTCCAAACATCTTCAGCTTTGCCGTGATCGATGCCACCTGCTCAGCCCC TCTGACTCTCATTCTTCCACCCCATCAGGAAAGCCCCCTTCCTGCAAACTTGTGCACCCGCCACTCTACC AGAAAAAATGCACCCTCATGGAATTTCTCTCACCCCTTTGGAATTTTGCTCAATGAGTTTATCTGAATTG CTTAAGGCTTTTAACAGCGCCAGCATCAGATGGAGAGCTAATCAACCCTGACATCAGCAAGACATTCTTG ATTTAAAGCTCTCAGCTAAATCTGCATCCATTAATCATTTTAGTTAACTATCTTGTTTGGCTTGATTGAG CTTGATGGTGTGAACATTACAAAGAGGTATTTTGTTTTTTACAGTCAAGTGTATATAAATAATCCAATTC TTATAAATTGGTTACTCAAATCATGCTAATAGAAAAGACTTTGAAGGTTTATTTTAAATGCTTCTCTTAT GGTTCCAAATAAAAACATTCCATAACTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5040944 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5040944 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17157 same embryo
	EMAGE:17158 same embryo
	EMAGE:17156 same embryo
	EurExpress:euxassay_001542 same experiment
	MGI:4823951 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source