EMAGE mouse gene expression spatial database

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EMAGE:17156

Dync1li1 dynein cytoplasmic 1 light intermediate chain 1 ( MGI:2135610)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001544_01	euxassay_001544_02	euxassay_001544_03	euxassay_001544_04	euxassay_001544_05

euxassay_001544_06	euxassay_001544_07	euxassay_001544_08	euxassay_001544_09	euxassay_001544_10

euxassay_001544_11	euxassay_001544_12	euxassay_001544_13	euxassay_001544_14	euxassay_001544_15

euxassay_001544_16	euxassay_001544_17	euxassay_001544_18	euxassay_001544_19	euxassay_001544_20

euxassay_001544_21	euxassay_001544_22	euxassay_001544_23	euxassay_001544_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17156_wholemount_strong.wlz

17156_wholemount_moderate.wlz

17156_wholemount_weak.wlz

17156_wholemount_possible.wlz

17156_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17156_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4741
Entity Detected:	Dync1li1, dynein cytoplasmic 1 light intermediate chain 1 ( MGI:2135610)
Sequence:	sense strand is shown >T4741 CGCCGCTTCGCTCTTCCAGCCCTGTCAGGAGTGGTGTGATTCCCGACCAAGATGGCGGCCGTGGGGCGAG TCGGCTCGTTCGGTTCATCTCCACCCGGGCTAGCCTCGACCTACGCCAGCGGACCCCTAGCCAACGAGCT AGCGTCGGGCAGCGGCGGCCCCGCGGCGGGCGACGATGAGGACGGGCAGAACCTTTGGTCCTGCATCCTC AGCGAGGTCTCCACGCGCTCGCGCTCCAAGCTCCCGACCGGGAAGAACGTGCTGCTGCTGGGTGAAGATG GAGCTGGGAAGACAAGCCTCATAAGAAGAATTCAGGGCATAGAGGAGTACAAGAAGGGAAGAGGACTGGA GTACCTGTACTTAAATGTGCACGACGAAGACAGGGATGATCAAACAAGATGTAATGTATGGATCTTAGAT GGAGATCTGTACCACAAAGGGCTACTCAAGTTCTCTCTGGATGCCCTTTCTCTGAGGGACACTCTGGTCA TGTTGGTTGTTGATATGTCAAAGCCTTGGACTGCTTTGGATTCCTTACAGAAATGGGCAAGTGTGGTGAG AGAACATGTCGACAAGCTGAAAATCCCCCCTGAAGAGATGAAGGAAATGGAGCAGAAGTTGATTAGAGAC TTCCAAGAGTACGTGGAGCCAGGAGAAGACTTCCCAGCCTCCCCTCAGCGAAGAACCACTGGTGCGCAGG AGGACAGAGGTGACAGTGTTGTCCTGCCCCTGGGTGCAGACACACTCACACACAACCTGGGCCTACCAGT GCTCGTAGTCTGTACGAAGTGTGATGCCATTAGTGTATTGGAGAAAGAGCATGACTACAGAGATGAACAC TTCGATTTTATCCAGTCTCACATCCGCAAGTTCTGTTTACAGTATGGCGCAGCGCTGATTTACACTTCAG TAAAAGAGAACAAGAACATAGACTTAGTTTATAAATACATCGTCCAGAAGCTGTACGGGTTCCCGTACAA GATCCCTGCGGTGGTGGTGGAGAAGGACGCAGTGTTTATTCCAGCAGGGTGGGATAATGATAAGAAAATA GGAATATTACATGAAAATTTTCAGACTTTGAAAGTAGAAGATAATTTTGAAGACATCATAACCAAACCTC CTGTCAGAAAGTTCGTGCATGAGAAGGAGATCATGGCAGAAGATGACCAAGTGTTTCTGATGAAGCTACA GTCTCTTTTAGCAAAGCAACCTCCAACTGCAGCTGGAAGGCCTGTGGATGCATCACCAAGAGTCCCTGGA GGCTCCCCTCGAACACCAAACAGATCTGTGTCATCCAATGTTGCCAGTGTGTCCCCCATCCCTGCTGGAT CCAAAAAAATTGATCCAAACATGAAAGCTGGAGCGACCAGCGAAGGGGTCCTGGCGAATTTCTTCAACAG TCTGTTGAGTAAGAAGACTGGCTCTCCTGGAGGCCCCGGGGTCGGCGGCAGTCCTGGAGGAGGGGCTGCA GGTGCAAGCCCCAGTTTGCCACCGTCCGCCAAAAAGTCAGGCCAGAAACCTGTGCTTTCAGATGTTCACG CAGAGCTAGACAGAATCACACGGAAACCTGCTTCTGTTTCTCCTACAACACCTACATCTCCTACGGAAGG AGAAGCCTCCTGAAGATACCAAACAAAGCCATTTGTTCATTCTTCTGGGTAATGACAGCTTGCCTCTGCC CTGCCCTTCCAAGTTGGAACTACGATGGGGCCGGAAGCTTTTACAAACAGACAAAGTTTATGTCCTTATG TCCTTTTTTTTTTTTTTTTGGTGGCTGCCCATTTTTAAAAGAGGAACTACACAGAAGAAAGACAGGTTTT TTTTTTTTTTGGTTTTTCGAGACAGGATTTCTCTGTATAGCCCTGGCTGTCCCAGAACTCACTTTGTAGA CCAGGCTGGCCTCAAACTCAGAAATCCTCCTGCCTCTGCCTCCGGTGTGCGCCACCAGGACCGGCTAAGA AAGACAATTTTACATTATGTAGAATAACTGTTTGCATTGCCATTCTGCAAAATGAAGAAATTTTGATTTT TGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5040044 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5040044 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17157 same embryo
	EMAGE:17158 same embryo
	EMAGE:17155 same embryo
	EurExpress:euxassay_001544 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source