EMAGE mouse gene expression spatial database

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EMAGE:17159

Vnn1 vanin 1 ( MGI:108395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001550. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001550_01	euxassay_001550_02	euxassay_001550_03	euxassay_001550_04

euxassay_001550_05	euxassay_001550_06	euxassay_001550_07	euxassay_001550_08	euxassay_001550_09

euxassay_001550_10	euxassay_001550_11	euxassay_001550_12	euxassay_001550_13	euxassay_001550_14

euxassay_001550_15	euxassay_001550_16	euxassay_001550_17	euxassay_001550_18	euxassay_001550_19

euxassay_001550_20	euxassay_001550_21	euxassay_001550_22	euxassay_001550_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17159_wholemount_strong.wlz

17159_wholemount_moderate.wlz

17159_wholemount_weak.wlz

17159_wholemount_possible.wlz

17159_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17159_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 06 07 09 11 12 13 14 16 17 18

4th ventricle lateral recess

moderate

regional

moderate expression: see section 02 03 04 11 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4752
Entity Detected:	Vnn1, vanin 1 ( MGI:108395)
Sequence:	sense strand is shown >T4752 CCACGCGTCCGAGCATGGGCACGTCTTGGTGGCTGGCGTGTGCTGCAGCGTTTTCTGCCCTCTGTGTCTT AAAAGCCAGCTCGCTGGATACTTTCCTCGCGGCTGTTTACGAGCATGCTGTGATCCTGCCTAAGGACACC CTGTTGCCAGTGTCTCACAGTGAGGCTCTGGCATTAATGAACCAGAATCTGGACCTTCTGGAAGGAGCGA TCGTATCTGCAGCGAAGCAGGGTGCGCACATTATTGTGACTCCAGAAGATGGCATATACGGTGTGCGTTT CACCAGGGATACGATCTACCCATACCTGGAGGAGATCCCAGACCCTCAAGTAAACTGGATACCCTGTGAT AACCCTAAAAGATTTGGCTCTACCCCGGTGCAGGAGAGACTCAGCTGCTTGGCCAAGAACAACTCCATCT ATGTTGTGGCGAACATGGGAGACAAGAAGCCGTGTAACACCAGCGACTCTCACTGTCCACCTGACGGCAG ATTCCAGTACAACACTGATGTGGTGTTTGATTCCCAGGGTAAACTGGTTGCGAGATACCATAAGCAAAAC ATTTTCATGGGAGAAGATCAGTTCAATGTCCCCATGGAGCCTGAGTTTGTGACTTTCGACACCCCCTTTG GAAAGTTTGGCGTCTTCACCTGTTTCGATATTCTCTTCCATGATCCCGCTGTCACCCTGGTGACAGAATT CCAGGTGGACACCATACTGTTCCCAACCGCCTGGATGGACGTCCTTCCTCATTTGGCAGCCATTGAATTC CACTCAGCTTGGGCTATGGGCATGGGGGTCAATTTCCTAGCAGCTAATCTACATAATCCCTCGAGGAGAA TGACAGGAAGTGGTATCTATGCACCCGATTCTCCAAGGGTCTTTCACTACGACAGGAAGACCCAAGAAGG AAAACTCCTCTTCGCTCAGCTGAAATCCCACCCAATTCACTCCCCGGTGAACTGGACTTCCTATGCTAGC AGTGTAGAATCAACCCCAACCAAAACCCAGGAATTTCAGAGTATTGTCTTTTTTGATGAGTTTACCTTTG TGGAGCTCAAAGGGATCAAAGGAAATTACACTGTTTGCCAGAATGACCTCTGCTGTCACCTAAGCTACCA GATGTCTGAGAAGCGAGCAGATGAGGTTTATGCCTTTGGAGCCTTTGATGGGCTGCACACCGTGGAAGGG CAGTACTACCTACAGATCTGCATCCTGCTAAAATGTAAAACTACCAATTTACGCACCTGTGGTAGTTCAG TGGACACGGCTTTTACCAGGTTTGAAATGTTCTCGCTCAGCGGCACTTTTGGAACCCGGTATGTCTTCCC TGAAGTGTTGCTGAGTGAGGTCAAGCTCGCACCTGGGGAGTTTCAGGTGTCAAGTGATGGGCGCCTGGTT AGCCTGAAGCCAACCTCGGGACCTGTGTTAACCATCGGGCTCTTTGGGAGGTTGTATGGGAAGGACTGGG CATCCAATGCTTCCTCAGACTTCATAGCACACTCGCTGATAATAATGCTGATTGTGACGCCTATTATACA TTACTTGTGCTGATGGAATTTTTACATTTTTTATTTTATTTAGAAAATTTAAAATTGGTGGATGCAGAAA AAATAACTGTTTGTCAACAGTGGACTCGGGTGTAAGCAAATAAAGTGCCTCTTCTTTAGAAAAACATATG TACACCAGATACATTTCAGGAAAATTAATAAAACTTTGAGCATTGGAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5041670 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5041670 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17162 same embryo
	EMAGE:17160 same embryo
	EMAGE:17163 same embryo
	EMAGE:17161 same embryo
	EurExpress:euxassay_001550 same experiment
	MGI:4829165 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source