EMAGE mouse gene expression spatial database

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EMAGE:17160

Mapk3 mitogen-activated protein kinase 3 ( MGI:1346859)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001546_01	euxassay_001546_02	euxassay_001546_03	euxassay_001546_04	euxassay_001546_05

euxassay_001546_06	euxassay_001546_07	euxassay_001546_08	euxassay_001546_09	euxassay_001546_10

euxassay_001546_11	euxassay_001546_12	euxassay_001546_13	euxassay_001546_14	euxassay_001546_15

euxassay_001546_16	euxassay_001546_17	euxassay_001546_18	euxassay_001546_19	euxassay_001546_20

euxassay_001546_21	euxassay_001546_22	euxassay_001546_23	euxassay_001546_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17160_wholemount_strong.wlz

17160_wholemount_moderate.wlz

17160_wholemount_weak.wlz

17160_wholemount_possible.wlz

17160_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17160_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4751
Entity Detected:	Mapk3, mitogen-activated protein kinase 3 ( MGI:1346859)
Sequence:	sense strand is shown >T4751 CGGGAGGAGTGGAGATGGCGGCGGCGGCGGCGGCTCCGGGGGGCGGGGGCGGGGAGCCCAGGGGAACTGC TGGGGTCGTCCCGGTGGTCCCCGGGGAGGTGGAGGTGGTGAAGGGGCAGCCATTCGATGTGGGCCCACGC TACACGCAGCTGCAGTACATCGGCGAGGGCGCGTACGGCATGGTCAGCTCAGCTTATGACCACGTGCGCA AGACCAGAGTGGCCATCAAGAAGATCAGCCCCTTTGAGCATCAAACCTACTGTCAGCGCACGCTGAGGGA GATCCAGATCTTGCTGCGATTCCGCCATGAGAATGTTATAGGCATCCGAGACATCCTCAGAGCGCCCACC CTGGAAGCCATGAGAGATGTTTACATTGTTCAGGACCTCATGGAGACAGACCTGTACAAGCTGCTTAAAA GCCAGCAGCTGAGCAATGACCACATCTGCTACTTCCTCTACCAGATCCTCCGGGGCCTCAAGTATATACA CTCAGCCAATGTGCTGCACCGGGACCTGAAGCCTTCCAATCTGCTTATCAACACCACCTGCGACCTTAAG ATCTGTGATTTTGGCCTGGCCCGGATTGCTGACCCTGAGCACGACCACACTGGCTTTCTGACGGAGTATG TGGCCACACGCTGGTACCGAGCCCCAGAGATCATGCTTAATTCCAAGGGCTACACCAAATCCATCGACAT CTGGTCTGTGGGCTGCATTCTGGCTGAGATGCTCTCCAACCGGCCCATCTTCCCCGGCAAGCACTACCTG GACCAGCTCAACCACATTCTAGGTATCTTGGGTTCCCCATCCCAGGAGGACCTTAATTGCATCATTAACA TGAAGGCCCGAAACTACCTGCAGTCTCTGCCCTCGAAAACCAAGGTGGCTTGGGCCAAGCTCTTTCCTAA ATCTGACTCCAAAGCTCTTGACCTGCTGGACCGGATGTTAACCTTCAACCCAAACAAGCGCATCACAGTA GAGGAAGCGCTGGCTCACCCTTACCTGGAACAGTACTACGATCCGACAGATGAGCCAGTGGCCGAGGAGC CATTCACCTTCGACATGGAGCTGGATGACCTCCCCAAGGAGCGGCTGAAGGAGTTGATCTTCCAGGAGAC AGCCCGCTTCCAGCCAGGGGCGCCAGAGGGCCCCTAACAAGAACAGACACCCCTGTCCTTTTGGATCTGG TCCTGCTCCTACCTGCTCCTTCTCTGCAGATTGTTAGAAAGTGAACTTTGCTCAACCCAGACCCCGGCAG CCCAGGCTGGACCAAGGGTGGGCCTGGCCACCTTCTCTCACTTTGCTGGGGTCTCCTGCTTCAAGCAGGC TTCTCCCACTCCAATCCCCTGCCCCATCTCCCCATAGCCTGAGTGATGAGGTGGCCCCAGAGCTGATCTC TGCTGCTGTGTCTTTATCTACCCTTGCTAGCCCCGGCTCTGGTAGACCGTTCTGGAATGGAGGGGCTATG ATCGTCCTAGGACCTGTGCTACGGAGGGGTGGAGGGCACTGAGTAGGGCTCTGCCCTATTCATCTTGTTG GAACCCCACCCCATTTTCCCTGACAGAACATTCCTAAGTCTCAAGGGCTAGTTTCCCTGAGGAGCCCAGG CCTAACCCTCTCTCTCTCAAGCTGCCACATGTAACGCCCTTGCTGCTTCTGTGTGTGGGTGATTGGATGT GGGGGTGGGGCCCGTGGAGAGCCGGTGCCCCTCCCCATCTCCCCGTGCCTGTATCTAATATATAAATATA GAGATGTGTATATGGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5041517 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5041517 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17162 same embryo
	EMAGE:17163 same embryo
	EMAGE:17161 same embryo
	EMAGE:17159 same embryo
	EurExpress:euxassay_001546 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source