EMAGE mouse gene expression spatial database

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EMAGE:17161

Slco1a6 solute carrier organic anion transporter family, member 1a6 ( MGI:1351906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001548. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001548_01	euxassay_001548_02	euxassay_001548_03	euxassay_001548_04

euxassay_001548_05	euxassay_001548_06	euxassay_001548_07	euxassay_001548_08	euxassay_001548_09

euxassay_001548_10	euxassay_001548_11	euxassay_001548_12	euxassay_001548_13	euxassay_001548_14

euxassay_001548_15	euxassay_001548_16	euxassay_001548_17	euxassay_001548_18	euxassay_001548_19

euxassay_001548_20	euxassay_001548_21	euxassay_001548_22	euxassay_001548_23	euxassay_001548_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

17161_wholemount_strong.wlz

17161_wholemount_moderate.wlz

17161_wholemount_weak.wlz

17161_wholemount_possible.wlz

17161_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:17161_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

regional

strong expression: see section 06 07 08 09 10 12 14 16 17 18

4th ventricle lateral recess

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4753
Entity Detected:	Slco1a6, solute carrier organic anion transporter family, member 1a6 ( MGI:1351906)
Sequence:	sense strand is shown >T4753 GCTTTAAACAATCAGAAGAACAAAATGGGAGAACCTGAGAAAAGGGTTGGAATCCACAGGGTCAGGTGCT TTGCCAAGATCAAGGTGTTTCTGTTGGCATTAATATGGGCATATATATCCAAAATACTATCAGGAGTTTA CATGAGTACTATGCTCACACAATTAGAGAGACAATTCAATATTTCCACATCTATAGTTGGACTTATCAAT GGGAGCTTTGAGATGGGTAACCTTTTGGTGATTGTATTCGTGAGTTATTTTGGAACAAAACTGCATAGAC CTATCATGATTGGTGTTGGTTGTGCAGTTATGGGCCTAGGGTGTTTCATAATATCACTACCTCATTTCCT CATGGGCAGATACGAATATGAAACAACGATTTCACCTACAAGCAACTTGTCCTCAAACAGCTTTTTGTGT GTGGAAAACAGATCCCAGACCTTAAAGCCAACACAAGACCCAGCAGAGTGTGTGAAAGAAATTAAATCAT TAATGTGGATATATGTACTGGTAGGAAACATTATACGTGGAATTGGTGAAACTCCCATCATGCCTTTAGG TATTTCCTATATAGAAGACTTTGCCAAATCAGAAAATTCTCCTTTATACATTGGAATTTTAGAAGTTGGG AAGATGATTGGCCCAATACTTGGATATTTGATGGGACCTTTCTGTGCAAACATTTATGTAGACACAGGGT CTGTGAATACAGATGACCTGACCATAACTCCCACTGATACACGATGGGTCGGTGCTTGGTGGATTGGCTT TTTGGTCTGTGCAGGAGTGAATGTCCTGACCAGCATCCCCTTTTTCTTCTTTCCAAAAACACTCCCAAAG GAAGGATTACAGGATAATGGGGATGGAACTGAAAATGCCAAAGAGGAGAAGCACAGAGACAAGGCCAAGG AGGAAAACCAAGGAATCATTAAAGAATTCTTCCTTATGATGAAGAACCTCTTCTGTAACCCTATTTACAT GCTTTGCGTCCTTACAAGTGTGCTCCAGGTAAATGGAGTTGCCAATATTGTGATTTACAAGCCTAAATAC CTGGAACATCATTTTGGAATCTCCACAGCAAAGGCAATCTTCCTTATTGGTCTTTATACCACACCTTCAG TATCTGCTGGATATTTAATTAGTGGTTTTATTATGAAGAAGTTGAAGATTACTCTCAAGAAAGCTGCAAT CATAGCACTTTGCCTATTCATGTCTGAGTGCCTTTTATCCCTTTGTAACTTTATGCTAACCTGTGATACC ACTCCAATTTCCGGCTTAACTACCTCTTATGAAGGAATTCAGCAGTCTTTTGATATGGAGAATAAGTTTC TTTCTGACTGCAACACAAGGTGTAACTGCTTAACAAAAACATGGGATCCAGTGTGTGGGGACAATGGCCT AGCATACATGTCACCTTGCCTTGCAGGCTGTGAAAAGTCTGTTGGAACAGGAGCCAACATGGTGTTTCAA AATTGCAGCTGCATTCGGTCATCAGGAAACTCATCTGCAGTCCTGGGGCTGTGTAAGAAAGGCCCTGACT GTGCTAACAAGCTTCAGTACTTTTTAATCATAACGGTATTTTGCTGCTTCTTCTACTCGTTAGCAACCAT ACCTGGGTACATGGTTTTTCTGAGATGTATGAAGTCTGAAGAGAAGTCACTTGGAATTGGATTACAGGCA TTTTTCATGAGACTATTTGCTGGTATTCCTGCATCTATTTACTTTGGCGCTTTGATAGACAGAACATGCT TACATTGGGGAACTCTGAAATGTGGTGAGCCAGGAGCATGCAGGACCTATGAAGTCAGTAGTTTCAGGCG CCTCTATCTTGGATTGCCTGCAGCTCTAAGAGGATCAATCATTCTTCCTTCATTATTCATTCTAAGACTT ATCAGGAAACTCCAAATCCCTGGGGACACTGACTCTTCAGAAATTGAACTTGCAGAGACGAAGCCCACAG AGAAGGAAAGTGAGTGCACAGACATGCACAAAAGTCCTAAGGTCGAGAACGATGGAGAACTGAAAACTAA GCTGTAATGAGGTTTCTACTGGCCTATGCAAGGCCACGAACAGAATACTCATTTCATTTCCTTTGAATCA TAAGAGAAATAATAGGAACCCTCATCTTTAAGGACCTCAAAAGCTATTTTTCTCATTATAAAAATAATTA CTGATATTATTTTCAGAACTTCAGGGTAGCACTTAAGATTTTCCTAGTGAAGACTTTAATGGTGACCCCC ACCCTGGACTTTAAAAAGCCTTCGTTTTCAAAGAGCATTTTCTCTTTAAACTCAGTCAAAGGAAATGTGT GTTTCTTGCATATCTTCAAGTAGATTTCATTTCACTTAATTTCATTGAATTTACATTTCAATATTGGAGG TAATTAGAGCTGAAAGTATGCCTTCTGGTTGTGTCATATTGAAATAAATTGTTCAGATTCATCCTTTCCA TGTGCAAGGTGTCTGCATGTGTCTTTAACTCTTTGGGAGCTGTTATCTTTCTTTTCTCATTCTAGACTTT TGATGCTTCAGAGATTAGACTCTCACTAATGTGTCATCTCGTGTTTTCAATTCCCTCTTTCATTATTCAT GTCACATATTTGATCATTTTGTTTAGAACTCTGACAAATTTAAACAGGTTATTAAAACTCATGTAGAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5042082 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5042082 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:17162 same embryo
	EMAGE:17160 same embryo
	EMAGE:17163 same embryo
	EMAGE:17159 same embryo
	EurExpress:euxassay_001548 same experiment
	MGI:4828295 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source